For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Shelf Life: 12 months after date of delivery
Molecular Formula: C47H61N5O9S
Molecular Weight: 872.08 g/mol
Purity: > 95 % (HPLC)
Form: glass-like solid
Solubility: Chloroform, DCM, DMF, DMSO, THF
Dde Biotin-DBCO is suitable for the introduction of a biotin moiety to Azide-labeled biomolecules via Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction.
The biotinylated molecule can be selectively captured through conventional affinity biotin - streptavidin purification and released under mild buffer conditions with hydrazine solution for subsequent identification by mass spectrometry, ELISA, dot blot or Western blot applications.
Important Product Information
If possible, avoid reducing agents in reaction buffers, this can interfere with the azide stability.
General Procedure for Release
Incubate beads in 100 mM phosphate buffer solution (pH 7.4) containing 2%(v/v) hydrazine for 30 - 120 min at room temperature. Reference  might be used as starting point for the set up of your individual assay.
 Yang et al. (2013) Cleavable Trifunctional Biotin Reagents for Protein Labeling, Capture and Release. Chem. Commun. 49:5366.