Dibenzocyclooctyne (DBCO)* Agarose is suitable to covalently capture Azide-tagged biomolecules via Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC).
The proteins of interest need to be metabolically, enzymatically or chemically azide-tagged. Subsequently, the DBCO Agarose containing the covalently attached proteins can be washed with high stringency, virtually eliminating any non-specifically bound proteins.
*DBCO is also known as ADIBO (= Azadibenzocyclooctyne) or DIBAC (= Dibenzoazacyclooctyne)
