5-(3-Azidopropyl)-uridine-5'-triphosphate, Triethylammonium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C12H20N5O15P3 (free acid)
Molecular Weight: 567.23 g/mol (free acid)
Exact Mass: 567.02 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: clear aqueous solution
Concentration: 10 mM - 11 mM
Spectroscopic Properties: λmax 260 nm, ε 8.8 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Incorporation into RNA by T7 RNA polymerase-mediated in vitro transcription (up to 1400 bp tested). For a comprehensive protocol please refer to PP-501 or PP-501-Cy3/Cy5.
3'-Azide-Labeling of T7 promotor-containing oligonucleotides[1,2,3]
The resulting azide-functionalized RNA can subsequently be processed via Cu(I)-free (azide-DBCO) or Cu(I)-catalyzed (azide-alkyne) click chemistry that offers the choice
- to introduce a Biotin group for subsequent purification tasks (via DBCO-functionalized Biotin or Alkynes of Biotin, respectively)
- to introduce fluorescent group for subsequent microscopic imaging (DBCO-functionalized fluorescent dyes or Alkynes of fluorescent dyes, respectively)
- to crosslink the RNA to azide- or alkyne functionalized biomolecules e.g.proteins
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.
 Rao et al. (2012) Enzymatic incorporation of an azide-modified UTP analog into oligoribonucleotides for post-transcriptional chemical functionalization. Nature Protocols 7 (6):1097.
 Rao et al. (2012) Posttranscriptional chemical functionalization of azide-modified oligoribonucleotides by bioorthogonal click and Staudinger reactions. Chem. Commun. 48 (4):498.
 Savant et al. (2015) A versatile toolbox for posttranscriptional chemical labeling and imaging of RNA. Nucleic Acid Res. doi: 10.1093/nar/gkv903.