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5-Azidomethyl-uridine (5-AmU)

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CLK-063 10 mg 114,75 Add to Basket/Quote Add to Notepad
Structural formula of 5-Azidomethyl-uridine (5-AmU)
Structural formula of 5-Azidomethyl-uridine (5-AmU)

For research use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C

Shelf Life: 12 months after date of delivery

Molecular Formula: C10H13N5O6

Molecular Weight: 299.24 g/mol

CAS#: 24751-67-5

Purity: ≥ 95 % (HPLC)

Form: white to off-white powder

Solubility: DMSO, methanol

Spectroscopic Properties: λmax 260 nm, ε 9.3 L mmol-1 cm-1 (Methanol)

Applications:
RNA synthesis monitoring (potentially)

Description:
5-AmU (5-Azidomethyl-uridine) can potentially be used as a replacement for BrU (5-Bromo-uridine) or the copper-catalyst requiring 5-EU (5-Ethynyl-uridine) to measure de novo RNA synthesis in proliferating cells. 5-AmU is cell permeable and might incorporate into nascent RNA instead of its natural analog uridine. This assumption is based on the previously demonstrated suitability of 5-AmdU (5-Azidomethyl-2'-deoxyuridine) for DNA synthesis monitoring[1] as well as 5-AmUTP (5-Azidomethyl-UTP) for RNA synthesis monitoring[2].

The resulting azide-functionalized RNA can subsequently be detected via Cu(I)-catalyzed (CuAAC) or Cu(I)-free strain promoted (SPAAC) Click Chemistry that offers the choice to

  • introduce a Biotin group for subsequent purification tasks (via Alkynes of Biotin or DBCO-functionalized Biotin, respectivly)
  • introduce a fluorescent group for subsequent microscopic imaging (via Alkynes of fluorescent dyes or DBCO-functionalized fluorescent dyes, respectivly).

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Selected References:
[1] Neef et al. (2014) An azide-modified nucleoside for metabolic labeling of DNA. Chembiochem. 15 (6):789.
[1] Sawant et al. (2016) A versatile toolbox for posttranscriptional chemical labeling and imaging of RNA. Nucleic Acids Res. 44 (2):e16.