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Azide-PEG3-Biotin Conjugate

Cat. No. Amount Price (EUR) Buy / Note
CLK-AZ104P4-25 25 mg 91,40 Add to Basket/Quote Add to Notepad
CLK-AZ104P4-100 100 mg 313,70 Add to Basket/Quote Add to Notepad
CLK-AZ104P4-1000 1 g 1.293,10 Add to Basket/Quote Add to Notepad
Structural formula of Azide-PEG3-Biotin Conjugate
Structural formula of Azide-PEG3-Biotin Conjugate

For research use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C

Shelf Life: 12 months after date of delivery

Molecular Formula: C18H32N6O5S

Molecular Weight: 444.54 g/mol

CAS#: 875770-34-6

Purity: > 95 % (H NMR)

Form: solid

Color: off-white to grey

Solubility: Chloroform, DCM, DMF, DMSO, THF

Azide-PEG3-Biotin is suitable for the introduction of a biotin moiety to terminal Alkyne- and strained Alkyne (e.g. DBCO)-labeled biomolecules via Cu(I)-catalyzed Alknye-Azide (CUAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction, respectively.

The hydrophilic PEG3 linker reduces or eliminates aggregation and precipitation during the labeling process by increasing the hydrophilicity of the target molecule. It furthermore enhances the accessibility of the biotin moiety and thus the detection efficiency of the biotinylated molecule via fluorescent or HRP-labeled streptavidin or its affinity purification via streptavidin agarose.

Due to the extremely high affinity of biotin towards streptavidin (KD = 10-15 M), the biotinylated molecule/streptavidin-interaction is essentially irreversible under physiological conditions.

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