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Dde Biotin-Azide

Cat. No. Amount Price (EUR) Buy / Note
CLK-1136-10 10 mg 236,19 Add to Basket/Quote Add to Notepad
CLK-1136-100 100 mg 1.228,13 Add to Basket/Quote Add to Notepad
Structural formula of Dde Biotin-Azide
Structural formula of Dde Biotin-Azide

For research use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C

Shelf Life: 12 months after date of delivery

Molecular Formula: C32H53N7O8S

Molecular Weight: 695.87 g/mol

CAS#: 1802907-93-2

Purity: ≥ 95 % (HPLC)

Form: amorphous solid

Solubility: Chloroform, DCM, DMF, DMSO, THF

Description:
Dde Biotin-Azide is suitable for the introduction of a biotin moiety to terminal Alkyne- and strained Alkyne (e.g. DBCO)-labeled biomolecules via Cu(I)-catalyzed Alkyne-Azide (CUAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction, respectively.

The biotinylated molecule can be selectively captured through conventional affinity biotin - streptavidin purification and released under mild buffer conditions with hydrazine solution for subsequent identification by mass spectrometry, ELISA, dot blot or Western blot applications.

Important Product Information
If possible, avoid reducing agents in reaction buffers, this can interfere with the azide stability.

General Procedure for Release
Incubate beads in 100 mM phosphate buffer solution (pH 7.4) containing 2%(v/v) hydrazine for 30 - 120 min at room temperature. Reference [1] might be used as starting point for the set up of your individual assay.

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Selected References:
[1] Yang et al. (2013) Cleavable Trifunctional Biotin Reagents for Protein Labeling, Capture and Release. Chem. Commun. 49:5366.