For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Shelf Life: 12 months after date of delivery
Molecular Formula: C35H55N9O12S
Molecular Weight: 825.93 g/mol
Purity: > 95 % (HPLC)
Form: slightly yellow amorphous solid
Solubility: Chloroform, DCM, DMF, DMSO, THF
Photocleavable Biotin-Azide is suitable for the introduction of a biotin moiety to terminal Alkyne- and strained Alkyne (e.g. DBCO)-labeled biomolecules via Cu(I)-catalyzed Alkyne-Azide (CUAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction, respectively.
The biotinylated molecule can be selectively captured through conventional affinity biotin - streptavidin purification and released under mild buffer conditions with UV irradiation for subsequent identification by mass spectrometry, ELISA, dot blot or Western blot applications.
Important Product Information
If possible, avoid reducing agents in reaction buffers, this can interfere with the azide stability.
General Procedure for Release
References - might be used as starting point for the set up of your individual assay.
 Szychowski et al. (2010) Cleavable Biotin Probes for Labeling of Biomolecules via Azide-Alkyne Cycloaddition. J. Am. Chem. Soc. 132 (51):18351.
 Olejnik et al. (1995) Photocleavable Biotin Derivatives: A versatile approach for the isolation of biomolecules. Proc. Natl. Acad. Sci. 92 (16):7590.
 Kim et al. (2009) An Azido-Biotin Reagent for Use in the Isolation of Protein Adducts of Lipid-derived Electrophiles by Streptavidin Catch and Photorelease. Mol. Cell. Proteom. 8 (9):2080.
 Wang et al. (2010) Enrichment and Site Mapping of O-Linked and N-Acetylglucosamine by a Combination of Chemical/Enzymatic Tagging, Photochemical Cleavage and Electron Transfer Dissociation Mass Spectrometry. Mol. Cell. Proteom. 9 (1):153.
 Koopmans et al. (2012) A photocleavable affinity tag for the enrichment of alkyne-modified biomolecules. RSC Advances 2:2244.