» Sign in / Register

Photocleavable Biotin-Azide

PC Biotin-Azide

Cat. No. Amount Price (EUR) Buy / Note
CLK-1119-10 10 mg 236,19 Add to Basket/Quote Add to Notepad
CLK-1119-100 100 mg 1.228,13 Add to Basket/Quote Add to Notepad
Structural formula of Photocleavable Biotin-Azide
Structural formula of Photocleavable Biotin-Azide

For research use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C

Shelf Life: 12 months after date of delivery

Molecular Formula: C35H55N9O12S

Molecular Weight: 825.93 g/mol

Purity: > 95 % (HPLC)

Form: slightly yellow amorphous solid

Solubility: Chloroform, DCM, DMF, DMSO, THF

Description:
Photocleavable Biotin-Azide is suitable for the introduction of a biotin moiety to terminal Alkyne- and strained Alkyne (e.g. DBCO)-labeled biomolecules via Cu(I)-catalyzed Alkyne-Azide (CUAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction, respectively.

The biotinylated molecule can be selectively captured through conventional affinity biotin - streptavidin purification and released under mild buffer conditions with UV irradiation for subsequent identification by mass spectrometry, ELISA, dot blot or Western blot applications.

Important Product Information
If possible, avoid reducing agents in reaction buffers, this can interfere with the azide stability.

General Procedure for Release

  • Resuspend the washed resin in 1 ml PBS and transfer them to a clear glass vial or quartz cuvette with a tight fitting cap
  • Irridate the suspension with UV light at 345 -375 nm for 1 h under constant agitation (e.g. using a hand held long wave UV lamp such as UVGL-25)
  • Subsequently agitate the sample at 37°C for 1 h. Avoid using a stir bar as this can crush some resins
  • Collect the eluant by centrifugation or gravity flow
  • To increase the recovery rate add PBS to the resin and incubate for an additional 2 -16 h or use a buffer containing 0.1-1% detergent and/or 0,25 -1 M NaCl


References [1]-[5] might be used as starting point for the set up of your individual assay.

Related products:

Selected References:
[1] Szychowski et al. (2010) Cleavable Biotin Probes for Labeling of Biomolecules via Azide-Alkyne Cycloaddition. J. Am. Chem. Soc. 132 (51):18351.
[2] Olejnik et al. (1995) Photocleavable Biotin Derivatives: A versatile approach for the isolation of biomolecules. Proc. Natl. Acad. Sci. 92 (16):7590.
[3] Kim et al. (2009) An Azido-Biotin Reagent for Use in the Isolation of Protein Adducts of Lipid-derived Electrophiles by Streptavidin Catch and Photorelease. Mol. Cell. Proteom. 8 (9):2080.
[4] Wang et al. (2010) Enrichment and Site Mapping of O-Linked and N-Acetylglucosamine by a Combination of Chemical/Enzymatic Tagging, Photochemical Cleavage and Electron Transfer Dissociation Mass Spectrometry. Mol. Cell. Proteom. 9 (1):153.
[5] Koopmans et al. (2012) A photocleavable affinity tag for the enrichment of alkyne-modified biomolecules. RSC Advances 2:2244.