For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Shelf Life: 12 months after date of delivery
Molecular Formula: C18H34N6O5
Molecular Weight: 414.50 g/mol
Purity: > 95 % (HPLC)
Form: colorless to slightly yellow oil
Solubility: DMSO, DMF, Methanol
Azide-PEG3-Desthiobiotin is suitable for the introduction of a desthiobiotin moiety to terminal Alkyne- and strained Alkyne (e.g. DBCO)-labeled biomolecules via Cu(I)-catalyzed Alknye-Azide (CUAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction, respectively.
The hydrophilic PEG3 linker reduces or eliminates aggregation and precipitation during the labeling process by increasing the hydrophilicity of the target molecule. It furthermore enhances the accessibility of the desthiobiotin moiety and thus the detection efficiency of the desthiobiotinylated molecule via fluorescent or HRP-labeled streptavidin or its affinity purification via streptavidin agarose.
Desthiobiotin binds less tightly to streptavidin (KD = 10-11 M) than Biotin (KD = 10-15M). Desthiobiotinylated molecules are therefore easily eluted from the complex in the presence of excess Biotin.