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Azide-PEG3-Desthiobiotin

Cat. No. Amount Price (EUR) Buy / Note
CLK-1107-10 10 mg 125,35 Add to Basket/Quote Add to Notepad
CLK-1107-100 100 mg 653,15 Add to Basket/Quote Add to Notepad
Structural formula of Azide-PEG3-Desthiobiotin
Structural formula of Azide-PEG3-Desthiobiotin

For research use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C

Shelf Life: 12 months after date of delivery

Molecular Formula: C18H34N6O5

Molecular Weight: 414.50 g/mol

CAS#: 1306615-47-3

Purity: > 95 % (HPLC)

Form: colorless to slightly yellow oil

Solubility: DMSO, DMF, Methanol

Description:
Azide-PEG3-Desthiobiotin is suitable for the introduction of a desthiobiotin moiety to terminal Alkyne- and strained Alkyne (e.g. DBCO)-labeled biomolecules via Cu(I)-catalyzed Alknye-Azide (CUAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction, respectively.

The hydrophilic PEG3 linker reduces or eliminates aggregation and precipitation during the labeling process by increasing the hydrophilicity of the target molecule. It furthermore enhances the accessibility of the desthiobiotin moiety and thus the detection efficiency of the desthiobiotinylated molecule via fluorescent or HRP-labeled streptavidin or its affinity purification via streptavidin agarose.

Desthiobiotin binds less tightly to streptavidin (KD = 10-11 M) than Biotin (KD = 10-15M). Desthiobiotinylated molecules are therefore easily eluted from the complex in the presence of excess Biotin.

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