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Azide Agarose

Azide Agarose is suitable to covalently capture terminal Alkyne and strained Alkyne (e.g DBCO)-tagged biomolecules via a Cu(I)-catalyzed Alkyne-Azide (CUAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction, respectively.

The proteins of interest need to be metabolically, enzymatically or chemically terminal Alkyne- or DBCO-tagged. Subsequently, the Azide Agarose containing the covalently attached proteins can be washed with high stringency, virtually eliminating any non-specifically bound proteins.