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2-Ethynyl-ATP (2-EATP)

2-Ethynyl-adenosine-5’-triphosphate, Sodium salt

Cat. No. Amount Price (EUR) Buy / Note
CLK-NU-004S 100 μl (10 mM) 112,75 Add to Basket/Quote Add to Notepad
CLK-NU-004L 5 x 100 μl (10 mM) 330,05 Add to Basket/Quote Add to Notepad
Structural formula of 2-Ethynyl-ATP (2-EATP) (2-Ethynyl-adenosine-5’-triphosphate, Sodium salt)
Structural formula of 2-Ethynyl-ATP (2-EATP)

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.

Shelf Life: 12 months

Molecular Formula: C12H16N5O13P3

Molecular Weight: 531.20 g/mol

Purity: ≥ 90 % (HPLC), contains approx. 6 % 2-Ethynyl-ADP

Form: sterile clear aqueous solution, in 100 mM Tris-HCl pH 7.5

Concentration: 10 mM - 11 mM

Spectroscopic Properties: λmax 265 nm; ε 10.6 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
in vitro polyadenylation of RNA[1]

Description:
2-Ethynyl-labeled adenosine triphosphate (2-EATP) is suitable for in vitro polyadenylation of RNA with recombinant poly(A) polymerase[1].

The resulting Alkyne-functionalized RNA can subsequently be processed via Cu(I)-catalyzed Azide-Alkyne click
chemistry (CUAAC) that offers the choice

  • to introduce a Biotin group for subsequent purification tasks (via Azides
    of Biotin)
  • to introduce fluorescent group for subsequent microscopic imaging (via
    Azides of fluorescent dyes)
  • to crosslink the RNA to azide-functionalized biomolecules e.g.proteins

Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

Related products:

Selected References:
[1] Curanovic et al. (2013) Global profiling of stimulus-induced polyadenylation in cells using a poly (A) trap. Nat. Chem. Biol. 9:671.
[2] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
[3] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.