For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C12H13N5O4
Molecular Weight: 291.26 g/mol
Exact Mass: 291.10 g/mol
Purity: ≥ 95 % (HPLC)
Solubility: up to 4.0 mM in 1 x PBS
mRNA poly(A) tail synthesis monitoring
2-Ethynyl-labeled adenosine (2-EA) can be used to measure de novo mRNA poly(A) tail synthesis in proliferating cells. 2-EA is cell permeable and incorporates into nascent mRNA transcripts both transcriptionally by RNA polymerase I,II and III and posttranscriptionally by poly(A) polymerase instead of their natural analolg adenosine.
The resulting ethynyl-functionalized RNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging.
Presolski et al. and Hong et al. provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.
 Curanovic et al. (2013) Global profiling of stimulus-induced polyadenylation in cells using a poly (A) trap. Nat. Chem. Biol. 9 (11):671.
 Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
 Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.