2-Ethynyl-adenosine
For general laboratory use.
Description:
2-Ethynyl-labeled adenosine (2-EA) can be used to measure de novo mRNA poly(A) tail synthesis in proliferating cells. 2-EA is cell permeable and incorporates into nascent mRNA transcripts both transcriptionally by RNA polymerase I,II and III and posttranscriptionally by poly(A) polymerase instead of their natural analolg adenosine.
The resulting ethynyl-functionalized RNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging[1].
Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C12H13N5O4
Molecular Weight: 291.26 g/mol
Exact Mass: 291.10 g/mol
Purity: ≥ 95 % (HPLC)
Form: solid
Color: white to off-white
Solubility: up to 4.0 mM in 1 x PBS
Applications:
mRNA poly(A) tail synthesis monitoring[1]
Related products:
- 2-Ethynyl-ATP (2-EATP), #CLK-NU-004
- 5-Ethynyl-uridine (5-EU), #CLK-N002
- Copper (II)-Sulphate (CuSO4), #CLK-MI004
- Tris(3-hydroxypropyltriazolylmethyl)amine (THPTA), #CLK-1010
- Sodium Ascorbate (Na-Ascorbate), #CLK-MI005
Selected References:
[1] Curanovic et al. (2013) Global profiling of stimulus-induced polyadenylation in cells using a poly (A) trap. Nat. Chem. Biol. 9 (11):671.
[2] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
[3] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.
| Cat. No. | Amount | Price |
|---|---|---|
| CLK-N005-1 | 1 mg | 103,90 € |
| CLK-N005-5 | 5 mg | 402,10 € |
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Scientific citations for Jena Bioscience clk-n005
5 scientific citations and 2 citation images found by Bioz.
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Influenza A virus derived NS1 enhances translation of HPLC purified mRNA and interferon adjuvanted mRNA vaccination
Scientific Reports, 2026-01-12 · PMID:41526569
All mRNAs used are 100% pseudouridine (Ψ)-modified.All mRNAs used are 100% pseudouridine (Ψ)-modified..
2-ethynyl adenosine (EA) and 5-ethynyl uridine (EU) were purchased fromJenabioscience . -
The nascent RNA labelling compound 5-ethynyl uridine (EU) integrates into DNA in some animals
BMC molecular and cell biology, 2025-12-26 · PMID:41454242
.. for 15 min in 50mM MgCl 2 in Nematostella medium.. Relaxed animals were then transferred to labelling medium containing 2% DMSO, 50mM MgCl 2 and either 0.5mM EU, 0.5mM EdU (5-ethynyl 2’-deoxyuridine, Invitrogen), 0.5mM EC (5-ethynyl cytidine, Jena Bioscience), 0.5mM EUTP (5-ethynyl UTP, Jena Bioscience) or 0.5mM EA (
2-ethynyl adenosine , Jena Bioscience).. In co-labelling assays, 0.1mM BrdU (5-Bromo-2 ́-deoxyuridine, Invitrogen) was combined .. -
The nascent RNA labelling compound 5-ethynyl uridine (EU) integrates into DNA in the sea anemone Nematostella vectensis
bioRxiv, 2024-10-22 · DOI:
PrePrint: .. for 15 min in 50mM MgCl 2 in Nematostella medium.. Relaxed animals were then transferred to labelling medium containing 2% DMSO, 50mM MgCl 2 and 0.5mM EU, 0.5mM EdU (5-ethynyl 2’-deoxyuridine, Invitrogen), 0.5mM EC (5-ethynyl cytidine, Jena Bioscience), 0.5mM EUTP (5-ethynyl UTP, Jena Bioscience) or 0.5mM EA (
2-ethynyl adenosine , Jena Bioscience).. All incubations were carried out for 2h at RT.All incubations ..
