For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
store dry and under inert gas
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C11H11FN2O5
Molecular Weight: 270.22 g/mol
Exact Mass: 270.07 g/mol
Purity: >95% (HPLC)
Color: off-white to grey
Solubility: Water, DMSO, DMF
F-ara-EdU [(2'S)-2'-deoxy-2'-fluoro-5-ethynyluridine] can be used as a replacement for EdU (5-ethynyl-2'-deoxyuridine) and BrdU (5-bromo-2'-deoxyuridine) to introduce bioorthogonal functional groups into DNA for diverse applications in basic research, biotechnology and drug discovery. Metabolic incorporation of F-ara-EdU into DNA can be detected by using Cu(I)-catalyzed click reaction with fluorescent azides. F-ara-EdU is less toxic than both BrdU and EdU, and it can be detected with greater sensitivity in experiments where long-term cell survival and/or deep-tissue imaging are desired.[1-2]
Presolski et al. and Hong et al. provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
 Neef and Luedtke et al. (2011) Dynamic metabolic labeling of DNA in vivo with arabinosyl nucleosides. Proc. Natl. Acad. Sci. U.S.A 108 (51):20404.
 Sorger et al. (2020) Torin2 Exploits Replication and Checkpoint Vulnerabilities to Cause Death of PI3K-Activated Triple-Negative Breast Cancer Cells. Cell Syst. 10 (1) :66.
 Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
 Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.