For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Shelf Life: 12 months after date of delivery
Molecular Formula: C21H35N3O6S
Molecular Weight: 457.58 g/mol
Purity: > 90 % (H NMR)
Form: white powder
Solubility: Chloroform, DCM, DMF, DMSO, THF
Acetylene-PEG4-Biotin is suitable for the introduction of a biotin moiety to Azide-labeled biomolecules via Cu(I)-catalyzed Alknye-Azide (CUAAC) reaction.
The hydrophilic PEG4 linker [26.4 Å (22 atoms)] reduces or eliminates aggregation and precipitation during the labeling process by increasing the hydrophilicity of the target molecule. It furthermore enhances the accessibility of the biotin moiety and thus the detection efficiency of the biotinylated molecule via fluorescent or HRP-labeled streptavidin or its affinity purification via streptavidin agarose.
Due to the extremely high affinity of biotin towards streptavidin (KD = 10-15 M), the biotinylated molecule/streptavidin-interaction is essentially irreversible under physiological conditions.