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Dde Biotin-Alkyne

Cat. No. Amount Price (EUR) Buy / Note
CLK-1137-1 1 mg 242,09 Add to Basket/Quote Add to Notepad
CLK-1137-25 25 mg 904,77 Add to Basket/Quote Add to Notepad
Structural formula of Dde Biotin-Alkyne
Structural formula of Dde Biotin-Alkyne

For research use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C

Shelf Life: 12 months after date of delivery

Molecular Formula: C32H50N4O8S

Molecular Weight: 650.83 g/mol

Purity: > 95 % (HPLC)

Form: glass-like solid

Solubility: Chloroform, DCM, DMF, DMSO, THF

Description:
Dde Biotin-Alkyne is suitable for the introduction of a biotin moiety to Azide-labeled biomolecules via Cu(I)-catalyzed Alkyne-Azide (CUAAC) Click Chemistry reaction.

The biotinylated molecule can be selectively captured through conventional affinity biotin - streptavidin purification and released under mild buffer conditions with hydrazine solution for subsequent identification by mass spectrometry, ELISA, dot blot or Western blot applications.

Important Product Information
If possible, avoid reducing agents in reaction buffers, this can interfere with the azide stability.

General Procedure for Release
Incubate beads in 100 mM phosphate buffer solution (pH 7.4) containing 2%(v/v) hydrazine for 30 - 120 min at room temperature. Reference [1] might be used as starting point for the set up of your individual assay.

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Selected References:
[1] Yang et al. (2013) Cleavable Trifunctional Biotin Reagents for Protein Labeling, Capture and Release. Chem. Commun. 49:5366.