For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Shelf Life: 12 months after date of delivery
Molecular Formula: C10H13N5O6
Molecular Weight: 299.24 g/mol
Purity: ≥ 95 % (HPLC)
Form: white to off-white powder
Solubility: DMSO, methanol
Spectroscopic Properties: λmax 260 nm, ε 9.3 L mmol-1 cm-1 (Methanol)
RNA synthesis monitoring (potentially)
5-AmU (5-Azidomethyl-uridine) can potentially be used as a replacement for BrU (5-Bromo-uridine) or the copper-catalyst requiring 5-EU (5-Ethynyl-uridine) to measure de novo RNA synthesis in proliferating cells. 5-AmU is cell permeable and might incorporate into nascent RNA instead of its natural analog uridine. This assumption is based on the previously demonstrated suitability of 5-AmdU (5-Azidomethyl-2'-deoxyuridine) for DNA synthesis monitoring as well as 5-AmUTP (5-Azidomethyl-UTP) for RNA synthesis monitoring.
The resulting azide-functionalized RNA can subsequently be detected via Cu(I)-catalyzed (CuAAC) or Cu(I)-free strain promoted (SPAAC) Click Chemistry that offers the choice to
 Neef et al. (2014) An azide-modified nucleoside for metabolic labeling of DNA. Chembiochem. 15 (6):789.
 Sawant et al. (2016) A versatile toolbox for posttranscriptional chemical labeling and imaging of RNA. Nucleic Acids Res. 44 (2):e16.