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Chemical CLICK-functionalization of recombinant proteins

CLICK-functionalized NHS Ester or Maleimides enable the introduction of an azide-, alkyne-, DBCO-, tetrazine- or trans-cyclooctene-group into recombinant proteins via targeting of lysine groups (NHS Ester-based Amine Labeling) or cysteine groups (Maleimide-based Thiol Labeling) (Tab. 1). The resulting randomly CLICK-functionalized protein can subsequently be processed via Cu(I)-catalyzed (azide-alkyne) or Cu(I)-free (azide-DBCO /tetrazine/TCO) click chemistry that offers the choice