For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Shelf Life: 12 months
Molecular Formula: C10H17N5O5
Molecular Weight: 287.27 g/mol
Purity: ≥ 95 % (NMR)
Form: white to off-white solid
Solubility: water, DMSO, MeOH
Legionella pneumophila specific metabolic lipopolysaccharide labeling
Legionaminic acid (LEG) is an essential lipopolysaccharide component exclusively synthesized by Legionella pneumophila.
The Azide-functionalized Legionaminic acid precursor pLEG-Azide (Man2NAc4NAc6-N3) provides an attractive approach for the specific detection of Legionella pneumophila in living cultures: It is cell-permeable, intracellularly processed and incorporated instead of its natural counterpart.
The resulting Azide-functionalized lipopolysaccharides can subsequently be detected via Cu(I)-catalyzed or Cu(I)-free Click Chemistry that offers the choice to introduce a Biotin group (via Alkynes of Biotin or DBCO-containing Biotin, respectively) for subsequent purification tasks or to introduce fluorescent group (via Alkynes of fluorescent dyes or DBCO-containing fluorescent dyes, respectively) for subsequent microscopic imaging.
Tested Legionella pneumophila strains:
Legionella pneumophila SG1 (Paris), Legionella pneumophila SG1 (Lens), Legionella pneumophila SG1 (Philadelphia), Legionella pneumophila SG3, Legionella pneumophila SG4, Legionella pneumophila SG5, Legionella pneumophila SG6
 Mas et al. (2014) Identification of Living Legionella pneumophila using species-specific metabolic lipopolysaccharide Labeling. Angew. Chem. Int. Ed. 53:1275.