pLEG-N3
Man2NAc4NAc6-N3
6-Azido-2,4-diacetamido-2,4,6-trideoxy-D-mannose
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| CLK-079 | 2,5 mg | 195,00 | Add to Basket/Quote Add to Notepad |
For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Shelf Life: 12 months after date of delivery
Molecular Formula: C10H17N5O5
Molecular Weight: 287.27 g/mol
CAS#: 1447950-85-7
Purity: ≥ 95 % (NMR)
Form: white to off-white solid
Solubility: water, DMSO, MeOH
Applications:
Legionella pneumophila specific metabolic lipopolysaccharide labeling
Description:
Legionaminic acid (LEG) is an essential lipopolysaccharide component exclusively synthesized by Legionella pneumophila.
The Azide-functionalized Legionaminic acid precursor pLEG-Azide (Man2NAc4NAc6-N3) provides an attractive approach for the specific detection of Legionella pneumophila in living cultures: It is cell-permeable, intracellularly processed and incorporated instead of its natural counterpart.
The resulting Azide-functionalized lipopolysaccharides can subsequently be detected via Cu(I)-catalyzed or Cu(I)-free Click Chemistry that offers the choice to introduce a Biotin group (via Alkynes of Biotin or DBCO-containing Biotin, respectively) for subsequent purification tasks or to introduce fluorescent group (via Alkynes of fluorescent dyes or DBCO-containing fluorescent dyes, respectively) for subsequent microscopic imaging.
Tested Legionella pneumophila strains[1]:
Legionella pneumophila SG1 (Paris), Legionella pneumophila SG1 (Lens), Legionella pneumophila SG1 (Philadelphia), Legionella pneumophila SG3, Legionella pneumophila SG4, Legionella pneumophila SG5, Legionella pneumophila SG6
Selected References:
[1] Mas et al. (2014) Identification of Living Legionella pneumophila using species-specific metabolic lipopolysaccharide Labeling. Angew. Chem. Int. Ed. 53:1275.
