For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at 4 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
The CuAAC Cell Reaction Buffer Kit (THPTA based)is suitable to perform Copper (Cu(I))-catalyzed Azide-Alkyne Click chemistry reactions (CuAAC) with cells containing metabolically functionalized Alkyne- or Azide modified biomolecules.
1 Kit provides sufficient amounts to perform 50 CuAAC experiments à 500 μl using 2 mM CuSO4 (copper source), 10 mM THPTA (Cu(I)-stabilizing ligand) and 100 mM Na-Ascorbate (reduction reagent) in 100 mM Na-Phosphate reaction buffer.
2 x 10 mg CuSO4 (M = 159.6 g/mol), #CLK-MI004)
Cu(I) stabilizing ligand:
5 x 25 mg THPTA (M= 434.5 g/mol, #CLK-1010)
4 x 200 mg Na-Ascorbate (M = 119.3 g/mol, #CLK-MI005)
2 x 30 ml sterile 100 mM Na-Phosphate Buffer, pH 7
10 ml sterile ddH2O
Materials required but not provided:
Alkyne-or Azide-functionalized substrates e.g. fixed and permeabilized cells containing metabolically functionalized Alkyne- or Azide-modified biomolecules.
(Picolyl)-Azide or Alkyne detection reagent and appropriate solvent (e.g. DMSO)
For labeling of fixed and permeabilized cells:
Washing solutions e.g. PBS containing 3% BSA
Fixation solution e.g. PBS containing 3.7% formaldehyd
Permeabilization solution e.g. PBS containing 0.5% Triton X-100
Mounting medium for imaging
Additional labeling reagent such as nuclear stain or antibody
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.
 Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
 Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.
 Besanceney-Webler et al. (2011) Increasing the Efficiacy of Bioorthogonal Click Reactions for Bioconjugation: A Comparative Study. Angew. Chem. Int. Ed. 50:8051.
 Uttamapinant et al. (2012) Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed. 51:5852.