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Click Reagents for Application on Gram-negative bacteria

Gram-negative bacteria are characterized by a lipopolysaccharide-containing outer cell membrane with 3-Deoxy-D-manno-octulosonic acid (KDO) as an essential component. The Azide-functionalized 3-Deoxy-D-manno-octulosonic acid (KDO-Azide) provides an attractive approach for the detection of gram-negative bacteria in living cultures[1]: It is cell-permeable, intracellularly processed and incorporated instead of its natural counterpart KDO.

Tested bacterial strains[1]:
Legionella pneumophila, Salmonella typhimurium, Escherichia coli O86, Escherichia coli K12

References

[1] Dumont et al. (2012) Click-Mediated Labeling of Bacterial Membranes through Metabolic Modification of the Lipopolysaccharide Inner Core. Angew. Chem. Int. Ed. 51:3143.