Gram-negative bacteria are characterized by a lipopolysaccharide-containing outer cell membrane with 3-Deoxy-D-manno-octulosonic acid (KDO) as an essential component. The Azide-functionalized 3-Deoxy-D-manno-octulosonic acid (KDO-Azide) provides an attractive approach for the detection of gram-negative bacteria in living cultures: It is cell-permeable, intracellularly processed and incorporated instead of its natural counterpart KDO.
Tested bacterial strains:
Legionella pneumophila, Salmonella typhimurium, Escherichia coli O86, Escherichia coli K12
 Dumont et al. (2012) Click-Mediated Labeling of Bacterial Membranes through Metabolic Modification of the Lipopolysaccharide Inner Core. Angew. Chem. Int. Ed. 51:3143.