2-Ethynyl-Adenosine-NAD⁺

For research use only!

Shipping: shipped on dry ice

Storage Conditions: store at -20 °C

Shelf Life: 12 months after date of delivery

Molecular Formula: C₂₃H₂₈N₇O₁₄P₂ (cation)

Molecular Weight: 688.46 g/mol (cation)

Form: clear aqueous solution

Concentration: 10 mM - 11 mM

Applications:
in vitro ADP-Ribosylation of proteins^{[1](compound 1)}
The resulting alkyne-functionalized protein^[1,2] can subsequently be processed via Cu(I)-catalyzed (azide-alkyne) click chemistry that offers the choice

• to introduce a Biotin group for subsequent purification tasks (via Azides of Biotin)
• to introduce fluorescent group for subsequent microscopic imaging (via Azides of fluorescent dyes)

Presolski et al.^[3] and Hong et al.^[4] provide a general protocol for Cu(I)-catalyzed click chemistry reactions and Lin et al.^[2] for ADP-Ribosylation assays that may be used as a starting point for the set up and optimization of individual assays.

Related products:

• Copper (II)-Sulphate (CuSO₄), #CLK-MI004
• Tris(3-hydroxypropyltriazolylmethyl)amine (THPTA), #CLK-1010
• Sodium Ascorbate (Na-Ascorbate), #CLK-MI005

Selected References:
[1] Wang et al. (2014) Chain-Terminating and Clickable NAD+ analogues for labeling the target proteins of ADP-Ribosyltransferases. Angew. Chem. Int. Ed. 53:8159.
[2] Lin et al. (2012) Labeling substrate proteins of Poly (ADP-ribose) polymerase with Clickable NAD analog. Curr. Prot. in Chem. Biol. 4:19.
[3] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
[4] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.