Interested in Bulk amounts of 7-Deaza-dGTP and/or N4-Methyl-dCTP?
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GC-rich DNA regions are still a challenge in amplification and sequencing applications due to the high thermal and structural stability of the G-C bond promoting complex secondary structure formation.
Strand-destabilizing nucleotide analogs such as 7-Deaza-dGTP and N4-Methyl-dCTP have been widely used to overcome such amplification and sequencing challenges of GC-rich DNA regions: They are enzymatically incorporated instead of their natural counterparts (dGTP or dCTP, respectively) thereby leading to enhanced amplification, improved resolution and reduced artifacts[1-5].
| Products | Cat. No. | Amount | Price |
|---|---|---|---|
| 7-Deaza-dGTP | NU-1179S | 10 µl (100 mM) | 155,40 € |
| NU-1179L | 5 x 10 µl (100 mM) | 454,90 € | |
| NU-1179-CSTM | Custom specific bulk amount | Request a Quote | |
| N4-Methyl-dCTP | NU-219S | 50 µl (100 mM) | 564,10 € |
| NU-219L | 5 x 50 µl (100 mM) | 2.467,40 € | |
| NU-219-CSTM | Custom specific bulk amount | Request a Quote |
Please contact Barbara with all questions or inquiries you may have!
Contact Barbara
[1] Peng et al. (2020) A novel target enrichment strategy in next-generation sequencing through 7-deaza-dGTP resistant enzymatic digestion. BMC Research Notes 13(445):https://doi.org/10.1186/s13104-020-05292-y.
[2] Motz et al. (2000) Improved Cycle Sequencing of GC-Rich Templates by a Combination of Nucleotide Analogs. BioTechniques 29:268.
[3] Flores-Juarez et al. (2016) PCR amplification of GC-rich DNA regions using the nucleotide analog N4-methyl-2'-deoxycytidine-5'-triphosphate. Biotechniques 61(4):175.
[4] Flores-Juarez et al. (2013) Capacity of N4-methyl-2'-deoxycytidine 5'-triphosphate to sustain the polymerase chain reaction using various thermostable DNA polymerases. Analytical Biochemistry 438(1):73.
[5] Li et al. (1993) Elimination of band compression in sequencing gels by the use of N4-methyl-2'-deoxycytidine 5' triphosphate. Analytical Biochemistry 21(11):2709.
