Black Hole Quencher™: True dark quenchers without native emission

Black Hole Quenchers™ (BHQs™) are the gold standard among quenchers in molecular biology, especially for quantitative PCR (qPCR) and other fluorescence-based assays.

Unlike traditional quenchers, BHQs™ are true dark quenchers without native emission, which reduces background noise and increases detection sensitivity.

Spectral properties and quenching range of Black Hole Quencher BHQ-1™, BHQ-2™ & BHQ-10™

Jena Bioscience now also offers BHQ-1™-, BHQ-2™- and BHQ-10™- labeled nucleotides to quench fluorescence across different spectral ranges (Table 1).

Table 1: Available BHQ™-nucleotide conjugates.

Nucleotide	Cat. No.	Quenching range	Recommended for quenching of e.g.
BHQ-1™
Aminoallyl-dUTP-BHQ-1	NU-803-BHQ1	480-580 nm	Cy3, Fluorescein & Rhodamine
5-Propargylamino-dCTP-BHQ-1	NU-809-BHQ1
7-Propargylamino-7-deaza-dATP-BHQ-1	NU-1611-BHQ1
7-Propargylamino-7-deaza-dGTP-BHQ-1	NU-1615-BHQ1
5-Propargylamino-ddUTP-BHQ-1	NU-1619-BHQ1
5-Propargylamino-ddCTP-BHQ-1	NU-850-BHQ1
7-Propargylamino-7-deaza-ddATP-BHQ-1	NU-1612-BHQ1
7-Propargylamino-7-deaza-ddGTP-BHQ-1	NU-1618-BHQ1
BHQ-2™
Aminoallyl-dUTP-BHQ-2	NU-803-BHQ2	560-670 nm	Cy5, AF594 & Texas Red
5-Propargylamino-dCTP-BHQ-2	NU-809-BHQ2
7-Propargylamino-7-deaza-dATP-BHQ-2	NU-1611-BHQ2
7-Propargylamino-7-deaza-dGTP-BHQ-2	NU-1615-BHQ2
5-Propargylamino-ddUTP-BHQ-2	NU-1619-BHQ2
5-Propargylamino-ddCTP-BHQ-2	NU-850-BHQ2
7-Propargylamino-7-deaza-ddATP-BHQ-2	NU-1612-BHQ2
7-Propargylamino-7-deaza-ddGTP-BHQ-2	NU-1618-BHQ2
BHQ-10™
Aminoallyl-dUTP-BHQ-10	NU-803-BHQ10	480-550 nm	AF488, Fluorescein & Rhodamine
5-Propargylamino-dCTP-BHQ-10	NU-809-BHQ10
7-Propargylamino-7-deaza-dATP-BHQ-10	NU-1611-BHQ10
7-Propargylamino-7-deaza-dGTP-BHQ-10	NU-1615-BHQ10
5-Propargylamino-ddUTP-BHQ-10	NU-1619-BHQ10
5-Propargylamino-ddCTP-BHQ-10	NU-850-BHQ10
7-Propargylamino-7-deaza-ddATP-BHQ-10	NU-1612-BHQ10
7-Propargylamino-7-deaza-ddGTP-BHQ-10	NU-1618-BHQ10

Please note: In addition to the brand-new Black Hole Quencher™, both ATTO-TEC (ATTO 612Q, 580Q & 540Q) and Dyomics (DYQ-661 & DYQ-660) quencher-labeled nucleotides are available off-the shelf and via a custom synthesis.

Questions or inquiries?

Please contact us for a quote if you are interested in BHQ™- labeled nucleotides!

Black Hole Quencher™: True dark quenchers without native emission

Table 1: Available BHQ™-nucleotide conjugates.

Questions or inquiries?

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