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New: Nuclease Control - As simple as this!

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Nucleases (e.g. RNases & DNases) are enzymes able to cleave phosphodiester bonds of nucleic acids (RNA, ss- or ds-DNA).

Due to their omnipresence in the environment, they pose a contamination risk especially to molecular biological applications such as PCR.

Nuclease Control Kits

Jena Bioscience improved its product line to detect lowest amounts of RNase A (0.2 pg/µl) and/or DNase I (1x10-5 units/µl) (Fig. 1). The master mixes are ready-to-go and come with an easy-to-use protocol for rapid simultaneous (multiplex) analysis based on labeled RNA- (FAM) and DNA probes (JOE) as reporter dyes.

The kits allow for excitation and detection with most common real-time PCR cyclers or fluorescence readers.

Fig. 1: Kinetic evaluation of DNase I and RNase A activity.

Fig. 1: A) Kinetic evaluation of DNase I activity monitored on the real-time PCR system QuantStudio 5 (ThermoFisher). Concentration of DNase I standards are 1 x 10-5/µl and 5 x 10-5/µl. PCR-grade water is used for negative control.
B)
Kinetic evaluation of RNase A activity monitored on the real-time PCR system QuantStudio 5 (ThermoFisher). Concentration of RNase A standards are 0.2 pg/µl and 1.0 pg/µl. PCR-grade water is used for negative control.


Contamination testing tools are available both for combined identification of RNase+DNase as well as of DNase by itself:

Product Cat. No. Amount Price
DNase Detection Kit PP-410S 100 reactions 163,68 €
PP-410L 500 reactions 654,72 €
RNase + DNase Detection Kit PP-409S 100 reactions 242,52 €
PP-409L 500 reactions 982,08 €
Dr. Buerk Schaefer

Require bulk scales or lyophilized formats? Get in touch with us regarding customized contamination control tests at pcr@jenabioscience.com !