Nucleases (e.g. RNases & DNases) are enzymes able to cleave phosphodiester bonds of nucleic acids (RNA, ss- or ds-DNA).
Due to their omnipresence in the environment, they pose a contamination risk especially to molecular biological applications such as PCR.
Jena Bioscience improved its product line to detect lowest amounts of RNase A (0.2 pg/µl) and/or DNase I (1x10-5 units/µl) (Fig. 1). The master mixes are ready-to-go and come with an easy-to-use protocol for rapid simultaneous (multiplex) analysis based on labeled RNA- (FAM) and DNA probes (JOE) as reporter dyes.
The kits allow for excitation and detection with most common real-time PCR cyclers or fluorescence readers.
Fig. 1: A) Kinetic evaluation of DNase I activity monitored on the real-time PCR system QuantStudio 5 (ThermoFisher). Concentration of DNase I standards are 1 x 10-5/µl and 5 x 10-5/µl. PCR-grade water is used for negative control.
B) Kinetic evaluation of RNase A activity monitored on the real-time PCR system QuantStudio 5 (ThermoFisher). Concentration of RNase A standards are 0.2 pg/µl and 1.0 pg/µl. PCR-grade water is used for negative control.
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