DNA amplification at constant temperature (Isothermal Amplification) offers key advantages over conventional PCR. Not only cost efficiency and simplicity are arguments for strand displacement techniques, also a high level of tolerance towards most PCR inhibitors makes this technology interesting for diagnostics, e.g. point-of-care applications worldwide.
Here we introduce a new innovative polymerase to reduce typical amplification times by a factor of 2-3 (Fig. 1). Saphir Bst2.0 Turbo benefits from an additional DNA-binding domain to ensure ultra-fast and robust amplification at 60 - 65 °C. Detect your target gene in just 5-10 min! Further, Saphir Bst2.0 Turbo has been developed to tolerate high salt concentrations for efficient direct analysis of blood samples or other difficult templates in diagnostics.
|Saphir Bst2.0 Turbo Polymerase||PCR-390S||2.000 units||118,50 €|
|PCR-390L||10.000 units||474,00 €|
|Bst Master Mixes|
|Saphir Bst2.0 Turbo GreenMaster||PCR-393S||2x 1,25 ml||128,25 €|
|PCR-393L||10x 1,25 ml||513,00 €|
|Saphir Bst2.0 Turbo GreenMaster highROX||PCR-394S||2x 1,25 ml||128,25 €|
|PCR-394L||10x 1,25 ml||513,00 €|
All master mixes are also available as lyophilisates.
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