The racing car among Bst Polymerases

DNA amplification at constant temperature (Isothermal Amplification) offers key advantages over conventional PCR. Not only cost efficiency and simplicity are arguments for strand displacement techniques, also a high level of tolerance towards most PCR inhibitors makes this technology interesting for diagnostics, e.g. point-of-care applications worldwide.

Here we introduce a new innovative polymerase to reduce typical amplification times by a factor of 2-3 (Fig. 1). Saphir Bst2.0 Turbo benefits from an additional DNA-binding domain to ensure ultra-fast and robust amplification at 60 - 65 °C. Detect your target gene in just 5-10 min! Further, Saphir Bst2.0 Turbo has been developed to tolerate high salt concentrations for efficient direct analysis of blood samples or other difficult templates in diagnostics.

Figure 1: Rapid isothermal amplification of target DNA (100 pg, 10 pg, 1 pg template) with Saphir Bst2.0 Turbo (blue; #PCR-390) compared to normal Saphir Bst2.0 (red, #PCR-389), Incubation at 65 °C.

Questions or inquiries?

All master mixes are also available as lyophilisates.

Get in touch for further information at pcr@jenabioscience.com!