DNA amplification at constant temperature (Isothermal Amplification) offers key advantages over conventional PCR. Not only cost efficiency and simplicity are arguments for strand displacement techniques, also a high level of tolerance towards most PCR inhibitors makes this technology interesting for diagnostics, e.g. point-of-care applications worldwide.
Here we introduce a new innovative polymerase to reduce typical amplification times by a factor of 2-3 (Fig. 1). Saphir Bst2.0 Turbo benefits from an additional DNA-binding domain to ensure ultra-fast and robust amplification at 60 - 65 °C. Detect your target gene in just 5-10 min! Further, Saphir Bst2.0 Turbo has been developed to tolerate high salt concentrations for efficient direct analysis of blood samples or other difficult templates in diagnostics.
Figure 1: Rapid isothermal amplification of target DNA (100 pg, 10 pg, 1 pg template) with Saphir Bst2.0 Turbo (blue; #PCR-390) compared to normal Saphir Bst2.0 (red, #PCR-389), Incubation at 65 °C.
Product | Cat. No. | Amount | Price |
---|---|---|---|
Bst Polymerase | |||
Saphir Bst2.0 Turbo Polymerase | PCR-390S | 2.000 units | 118,50 € |
PCR-390L | 10.000 units | 474,00 € | |
Bst Master Mixes | |||
Saphir Bst2.0 Turbo GreenMaster | PCR-393S | 2x 1,25 ml | 128,25 € |
PCR-393L | 10x 1,25 ml | 513,00 € | |
Saphir Bst2.0 Turbo GreenMaster highROX | PCR-394S | 2x 1,25 ml | 128,25 € |
PCR-394L | 10x 1,25 ml | 513,00 € |
All master mixes are also available as lyophilisates.
Get in touch for further information at pcr@jenabioscience.com!