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High Speed His-Tag Detection

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Want to detect His-tag fusion proteins
...in just 15 - 90 min time?
...with no need to apply a second antibody?
...without significant hands-on time?

Then read more below!

The His Detect Solution is a ready-to-use reagent for high speed detection of his-tagged proteins. It contains an optimized poly-histidine primary antibody conjugated to labeled gold nanoparticles for direct protein visualization. Staining occurs by simply adding the solution onto the blot and incubating for 15 to 90 min with NO need of second antibody. His-Detect is highly specific for C- and N-terminal His-tags and sensitive up to sub-picomole protein levels.

Application:

  • Detection of His-tagged proteins from samples in SDS-gel:
  • Detection of His-tagged proteins from samples in SDS-gel
  • Detection of fractions containing His-tagged proteins:
  • Detection of fractions containing His-tagged proteins
  • Expression level analysis of His-tagged proteins at the colony level:
  • Expression level analysis of His-tagged proteins at the colony level

Advantages:

  • Specific staining of His-tagged proteins → no need of blocking solutions
  • Highly Sensitive → detection of up to 0.1 pmol of protein
  • Efficient → results in 15 min to 90 minutes
  • One-step staining → ready-to-use solution with no need of additional washings
  • Economic → resusable solution
  • Visually detectable results → no readout equipment required

Dr. Buerk Schaefer

E-Mail me for technical inquiries or further information: pcr@jenabioscience.com

Reference:

[1] Cornvik et al. (2005): Colony filtration blot: a new screening method for soluble protein expression in Escherichia coli. Nat. Methods 2:507.