Traditional dyes such as Fluorescein or TAMRA are often hampered by low photostability and/or low hydrophilicity. Both however are critical parameter for detection sensitivity and bioconjugate solubility, respectively.
AZDyes (structural analogs to Alexa Fluor® dyes) are hydrophilic fluorescent dyes with remarkable photostability. We now offer both functionalized AZDyes and AZDye-nucleotide conjugates. CLICK AZDyes are routinely used for Click Chemistry – based labeling.
| CLICK AZDyes | AZDye - Nucleotide conjugates | ||||||||
| Dye | Substitute for |
Excmax [nm]** |
Emmax [nm]** |
εmax* | Azide | Picolyl-Azide | Alkyne | DBCO*** = ADIBO/DIBAC |
dUTP |
| AZDye488 (structural analog to Alexa Fluor®488) |
Cy2 FITC (Fluorescein) ATTO488 |
494 | 517 | 73 | ✔ | ✔ | ✔ | ✔ | ✔ |
| AZDye 546 (structural analog to Alexa Fluor®546) |
TAMRA | 554 | 570 | 110 | ✔ | ✔ | ✔ | ✔ | - |
| AZDye 555 (structural analog to Alexa Fluor®555) |
Cy3 ATTO550 |
555 | 572 | 155 | ✔ | ✔ | ✔ | ✔ | + |
| AZDye 594 (structural analog to Alexa Fluor®594) |
Texas Red | 590 | 617 | 92 | ✔ | ✔ | ✔ | ✔ | ✔ |
| AZDye 647 (structural analog to Alexa Fluor®647) |
Cy5 ATTO647N |
648 | 671 | 270 | ✔ | ✔ | ✔ | ✔ | + |
Can’t find the nucleotide-dye conjugate you are looking for? Just contact us!
