Freeze-dried/lyophilised PCR mixes are stable at ambient temperatures and allow point-of-care use without any refrigerating infrastructure (Fig. 1). Simply fill up vials with template DNA and run PCR as usual.
Fig. 1: Dry PCR mixes remain fully functional even after 12 months heat exposure (30 °C, green circle) while liquid mixes significantly lose activity even when stored in the fridge (4 °C, red circle). (Assay: Amplification of a 4 kb fragment of lambda DNA in a template dilution series down to 10 pg).
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