For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles, store in aliquots
Shelf Life: 12 months
Form: liquid (Supplied as serum)
Polyclonal Antibody pABahPML(460-479)#3 to human PML.
Indirect Immunofluorescence 1:1000 to 1:2000. Western Blot 1:1000
Antibody specificity was evalutated by indirect immunoflourescence and western blotting (see Figure 1A and B). The antibody detects all PML isoforms according to Condemine et al. (2006).
The antibody binds to a peptide corresponding to amino acids 460-479 (KGPSYGEDVSNTTTQKRKC) of the human PML protein. No cross reaction with mouse PML protein was observed in Western blots. The immunogen peptide sequence is not conserved in rat, chimpanzee, dog, or bovine PML proteins.
Krejcí et al. (2008) Nuclear organization of PML bodies in leukaemic and multiple myeloma cells. Leukemia Research 32:1866.
Condemine et al. (2006) Characterization of endogenous human promyelocytic leukemia isoforms. Cancer Res. 66:6192.