Protein farnesyltransferase mutant, α- and β-subunit
rat, recombinant, E. coli
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| PR-952 | 20 μg | 369,20 | Add to Basket/Quote Add to Notepad |
For general laboratory use.
Shipping: shipped on dry ice
Storage Conditions: store at -80 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: GST-α: 73 kDa, β: 48 kDa
Purity: > 90 % (SDS-PAGE)
Form: liquid (Supplied in 25 mM HEPES pH 7.2, 40 mM NaCl and 2 mM DTT)
Activity: 1 pmol of FTase double mutant will transfer 0.4 pmol of BGPP to H-Ras in 15 min at 37°C.
Description:
FTase is one of the three mammalian protein prenyltransferases. The double mutant of FTase was engineered to efficiently transfer biotin-geranylpyrophosphate (BGPP, Cat.# LI-015) onto its native protein substrates. This approach allows the detection of femtomolar amounts of prenylatable proteins in eukaryotic cells and tissues as well as effective inhibitor screening.
BIOZ Product Citations:
Selected References:
Hougland et al. (2009) Getting a handle on protein prenylation. Nat. Chem. Biol. 5 (4):197.
Nguyen et al. (2009) Analysis of the eukaryotic prenylome by isoprenoid affinity tagging. Nat. Chem. Biol. 5 (4):227.
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