AP₄U

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₁₉H₂₇N₇O₂₁P₄ (free acid)

Molecular Weight: 813.35 g/mol (free acid)

Exact Mass: 813.02 g/mol (free acid)

CAS#: 10527-48-7

Purity: ≥ 95 % (HPLC)

Form: solution in water

Color: colorless to slightly yellow

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_max 260 nm, ε 25.0 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Applications:
Synthesis by mutated enzyme H96G-Fhit^[1]
Formation by UTP-glucose-1-phosphate uridyltransferase^[2]
Synthesis by pyrococcus furiosus DNA ligase^[3]
TUNEL^[3]
Effector of Phosphotransferase^[4]
Inhibitor of Uridine Kinase^[5]
Vasoregulator through P2X₁, P2Y₂ and P2Y₄ receptors^[6]

BIOZ Product Citations:

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Selected References:
[1] Huang et al. (2004) Engineering human Fhit, a diadenosine triphosphate hydrolase, into an efficient dinucleoside polyphosphate synthase. J. Am. Chem. Soc. 126 (31):9548.
[2] Guranowski et al. (2004) Uridine 5'-polyphosphates (p4U and p5U) and uridine (5')polyphospho (5')nucleosides (Up (n)Ns) can be synthesized by UTP:glucose-1-phosphate uridylyltransferase from Saccharomyces cerevisiae. FEBS Lett. 561 (1-3):83.
[3] Günther et al. (2002) Thermostable Pyrococcus furiosus DNA lgase catalyzes the synthesis of (di)nucleoside polyphosphates. Extremophiles 6 (1):45.
[4] Marques et al. (1998) IMP-GMP 5'-nucleotidase from rat brain: activation by polyphosphates. J. Neurochem. 71 (3):1241.
[5] Cheng et al. (1986) Homogeneous uridine kinase from Ehrlich ascites tumor: substrate specificity and inhibition by bisubstrate analogs. Mol. Pharmacol. 30 (2):159.
[6] Jankowski et al. (2005) Uridine adenosine tetraphosphate: a novel endothelium- derived vasoconstrictive factor. Nature Medicine 11 (2):223.