γ-Aminophenyl-m7GTP (C10-spacer)-Agarose
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| AC-155S | 1 ml | 286,10 | Add to Basket/Quote Add to Notepad |
| AC-155L | 5 ml | 1.145,00 | Add to Basket/Quote Add to Notepad |
For general laboratory use.
Shipping: shipped at 4 °C
Storage Conditions: store at 4 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Applications:
Suitable for purification of eukaryotic mRNA cap-binding proteins.
Degree of substitution: 5 μmol - 7 μmol m7GTP/ml gel
Storage buffer: 20% Ethanol
BIOZ Product Citations:
Selected References:
Coots et al. (2017) m6A Facilitates eIF4F-Independent mRNA Translation. Mol. Cell. doi:10.1016.
Hou et al. (2017) PERK Signal-Modulated Protein Translation Promotes the Survivability of Dengue 2 Virus-Infected Mosquito Cells and Extends Viral Replication Viruses 9(9):262.
Theodosakis et al. (2017) p90RSK Blockade Inhibits Dual BRAF and MEK Inhibitor-Resistant Melanoma by Targeting Protein Synthesis. J. Invest. Dermatol. 137(10):2187.
Arimoto-Matsuzaki et al. (2016) TIA1 oxidation inhibits stress granule assembly and sensitizes cells to stress-induced apoptosis. Nat. Commun. 7:10252.
Cinti et al. (2016) HIV-1 Gag Blocks Selenite-Induced Stress Granule Assembly by Altering the mRNA Cap-Binding Complex mBio 7 (2):e00329-16.
Timpano et al. (2016) Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions. J. Vis. Exp. doi:10.3791.
Wahba et al. (2016) Polysome Profiling Links Translational Control to the Radioresponse of Glioblastoma Stem-like Cells. Cancer Res. 76 (10):3078.
Hatakeyama et al. (2014) A Novel Functional Site in the PB2 Subunit of Influenza A Virus Essential for Acetyl-CoA Interaction, RNA Polymerase Activity, and Viral Replication. J. of Biol. Chem. 289:24980.
Igreja et al. (2014) 4E-BPs require non-canonical 4E-binding motifs and a lateral surface of eIF4E to repress translation Nat. Commun. 5:4790.
Morino et al. (1996) Crystallization and preliminary X-ray diffraction study of recombinant human eukaryotic initiation factor-4E. J. Biochem. (Tokyo) 119 (2):224.
Webb et al. (1984) Purification of the messenger RNA cap-binding protein using a new affinity medium. Biochemistry 17,23 (2):177.
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