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| Please click here if this newsletter is not displayed properly! | ||||||
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| (1) JBSCREEN PACT++ & JBSCREEN JCSG++ | ||||||
JBScreen PACT++ is a systematic screen for pH, anion and cation testing in the presence of polyethylene glycol. The screen, developed by Newman et al., comprises 3 sub-screens each using a different PEG. PEG 1500 is screened against 4 broad range buffer systems at 6 distinct pH values. PEG 6000 is screened against 6 different anions while PEG 3350 is screened against 12 cations. This systematic approach helps to investigate the precipitation behaviour of the protein, even in the absence of crystals. In contrast, JBSreen JCSG++ is an optimized sparse matrix screen developed at the Joint Center for Structural Genomics (JCSG). 96 reagents have been handpicked with the aim to maximize the coverage of the crystallization parameter space and to reduce the redundancy of crystallization conditions within commercially available crystallization screens. When JBScreen PACT++ is used along with JBScreen JCSG++, systematic investigation of the precipitation behaviour of the protein can be combined with a sparse matrix screen in order to enhance the success rate of protein crystallization. http://www.jenabioscience.com/cms/en/1/catalog/1184/ (JBScreen PACT++) http://www.jenabioscience.com/cms/en/1/catalog/1134/ (JBScreen JCSG++)
[1] Newman et al. (2005) Towards rationalization of crystallization screening for small- to medium-sized academic laboratories: the PACT/JCSG+ strategy. Acta Cryst. D61:1426.
[2] Page et al. (2004) Shotgun crystallization strategy for structural genomics: an optimized two-tiered crystallization screen against the Thermotoga maritima proteome. Acta Cryst. D59:1028. |
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| (2) MICROMOUNTS™ AND MICROMESHES™ WITH PRECISE SPINE COMPATIBLE PIN LENGTH | ||||||
MiTeGen's MicroMounts™ and MicroMeshes™ are high performance tools for manipulating and mounting crystals. They are attached to solid stainless steel pins with standard lengths of 11, 19 and 25 mm. We are happy to announce that there are now available with SPINE standard pin length. For all sizes, the distance from the pin base to the center of the crystal aperture is precisely 18.5 mm with a length tolerance of 0.3 mm only. http://www.jenabioscience.com/cms/en/1/catalog/1187/ (MicroMounts™) http://www.jenabioscience.com/cms/en/1/catalog/1189/ (MicroMeshes™) for more information. |
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| (3) AXYGEN'S SYSTEMATICALLY CONTROLLED CRYSTALLIZATION SCREEN SET 101 | ||||||
This Protein Crystallization Screen has been designed by Armando Villaseñor and Seth Harris (Roche) expanding on the work by Tran et al. [1] to enable statistical evaluation of crystallization trials. The screen places emphasis on the three most efficient precipitants, i.e. Ammonium Sulfate, Malonate and PEG [2] both in isolation and in combination with a second precipitant.
[1] Tran et al. (2004) Statistical experimental design of protein crystallization screening revisited. Acta Cryst. D60:1562.
[2] Peat et al. (2005) Tapping the Protein Data Bank for crystallization information. Acta Cryst. D61:1662. http://www.jenabioscience.com/cms/en/1/catalog/1182/ |
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