|
||||||||||||||||||||||||||||||||
|
||||||||||||||||||||||||||||||||
| |
||||||||||||||||||||||||||||||||
| Please click here if this newsletter is not displayed properly! | ||||||||||||||||||||||||||||||||
Numerous GTP-analogs affect microtubule assembly in vitro. Jena Bioscience is glad to introduce a variety of purine nucleotides such as non-hydrolyzable, fluorescent mant-, 6-thio-, C2- and C8-modified derivatives that are promotive or inhibitory in microtubule assembly. Microtubule image is taken from Wikipedia, www.wikipedia.org. Properties of selected GTP-derivatives for microtubulin assembly:
|
||||||||||||||||||||||||||||||||
| NONHYDROLYZABLE GTP-ANALOGS | ||||||||||||||||||||||||||||||||
Normal microtubules will form in the presence of non-hydrolyzable GTP analogs (e.g. GpCpp [GMPCPP]) but the analogs prevent depolymerization. The "caged" version of non-hydrolyzable GTP analogs can be activated by flash photolysis with UV-light which leads to an "unmasking" of the biologically active nucleotide from the inactive caged species resulting in a rapid and highly localized release of the nucleotide. |
||||||||||||||||||||||||||||||||
| FLUORESCENT mant-GTP | ||||||||||||||||||||||||||||||||
These intrinsically fluorescent nucleotides combine fluorescence with close mimicry of the properties of natural nucleotides and may be used for fluorescent labeling of tubulin. Fluorescently-labeled tubulins have been utilized to detect both temporal and spacial changes of different classes of microtubules in various cell types and to investigate the dynamics of individual microtubules. The mant-derivatives of GTP may also be useful in determining the affinity of non-labeled nucleotide analogs for nucleotide-binding proteins. |
||||||||||||||||||||||||||||||||
| 6-THIO-GTP-DERIVATIVES | ||||||||||||||||||||||||||||||||
The introduction of a reactive thiol group into C6 position of GTP (6-thio-GTP) largely reduces the activity to support microtubulin assembly. When the reactivity of the thiol group is attenuated by substitution of hydrogen with a methyl group (forming 6-CH3-S-GTP) the ability to promote assembly is resumed. |
||||||||||||||||||||||||||||||||
| 8-Br-GTP | ||||||||||||||||||||||||||||||||
The prokaryotic tubulin homologue FtsZ (Filamentous temperature sensitive Z) plays a key role in bacterial cell division. Selective inhibitors of the GTP-dependent polymerization of FtsZ are promising candidates for new classes of antibacterial agents. 8-Bromoguanosine-5'-triphosphate (8-Br-GTP) acts as a competitive inhibitor of FtsZ polymerization but seems not to block eukaryotic tubulin assembly |
||||||||||||||||||||||||||||||||
| C2-MODIFIED PURINE NUCLEOTIDES (ITP, XTP) | ||||||||||||||||||||||||||||||||
The substitution of the amino group at position C2 of GTP with hydrogen or an oxo-group leads to formation of inosine triphosphate (ITP) or xanthosine triphosphate (XTP), respectively. These nucleotides are promotive in the assembly of microtubules with activity of ITP > GTP > XTP. http://www.jenabioscience.com/cms/en/1/catalog/740_tubulinassembling_nucleotides.html |
||||||||||||||||||||||||||||||||
|
||||||||||||||||||||||||||||||||
If you wish not to receive further email newsletters from us, please reply to this E-mail with the subject "UNSUBSCRIBE" or unsubscribe via the form at our website at http://www.jenabioscience.com.
|
||||||||||||||||||||||||||||||||
|
||||||||||||||||||||||||||||||||
| Responsible for content / Imprint | ||||||||||||||||||||||||||||||||
Jena Bioscience GmbH Loebstedter Str. 80 07749 Jena Germany Phone:+49 – 3641 – 6285 000 Fax: +49 – 3641 – 6285 100 E-Mail: info@jenabioscience.com Register Court: Amtsgericht Jena, HRB 207171 VAT No.: DE 195825742 Managing Directors: Thomas Billert Dr. Mathias Grün |
||||||||||||||||||||||||||||||||
| http://www.jenabioscience.com | ||||||||||||||||||||||||||||||||
