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Jena Bioscience is one of only a few suppliers that offers the nucleoside triphosphates ATP, CTP, GTP, and UTP of highest quality from milligram to kg scale. Every NTP lot is individually assayed regarding biophysical properties, purity by HPLC, and functional tests (see our NTP Solutions Specifications and NTP Lyophilisates Specifications) including
- absence of DNase, RNase and, Protease
- absence of nicking activity
- tested by T7 RNA Polymerase-mediated in vitro transcription
Especially the NTP performance in RNA in vitro transcription, is crucial for efficient synthesis of labeled probes or large amounts of unlabeled RNA[1,2] (Figure 1).
For more detailed information about our NTP lyophilisates please see:
www.jenabioscience.com/cms/en/1/catalog/2067
References
[1] Graeme L. Conn (Ed.) (2012) Recombinant and in vitro RNA synthesis. Methods in Molecular Biology 941, Springer Protocols, Humana Press.
[2] Roca et al. (2012) RNA biology in a test tube – an overview of in vitro systems/assays. Wiley Interdiscip. Rev. RNA 3(4):509.
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