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     Nourseothricin (NTC) – The Gold Standard antibiotic for selection in molecular genetics
   
   


(1) NTC – An efficient marker for broad spectrum of organisms
(2) NTC – Background and mechanism of action

   
       
  
   
   

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   (1) NTC – An efficient marker for broad spectrum of organisms
   


Nourseotricin (NTC, sometimes termed clonNAT) is an excellent selection antibiotic for genetic modification of a wide range of organisms including bacteria, yeast, fungi, protozoa, microalgae, and plant cells (Table 1). NTC is highly soluble in water and long-term stable as powder or solution. It shows no or very low background, no cross-reactivity and no cross-resistance with therapeutic antibiotics.

Group Species MIC*
µg/ml
Selection concentration
µg/ml
Gram-negative bacteria Agrobacterium tumefaciens
100
Escherichia coli 2-12 50
Francisella tularensis
50
Pseudomonas aeruginosa 50 100
Gram-positive bacteria Bacillus subtilis 5 50
Enterococcus faecium 8-256 500
Staphylococcus aureus 2-12 50
Streptomycetes Streptomyces lividans 6 100
Yeast Candida albicans 200 250-450
Hansenula polymorpha
100
Kluyveromyces lactis
50
Pichia pastoris
100
Saccharomyces cerevisiae 25 75-100
Schizosaccharomyces pombe 40 100
Other Ascomycota Acremonium chrysogenum
25
Aspergillus nidulans
120
Cryphonectria parasitica
100
Neurospora crassa
200
Penicillium chrysogenum
150-200
Podospora anserina
50
Sordaria macrospora
50
Trichophyton mentagrophytes
50
Basidiomycota Cryptococcus neoformans
100
Schizophyllum commune 3 8
Ustilago maydis
75-100
Protozoa Leishmania tarentolae, major etc.
100
Phytomonas serpens
100
Plasmodium falciparum 75**
Toxoplasma gondii
500
Microalgae Phaeodactylum tricornutum
50-250
Thalassiosira pseudonana
100
Plants Arabidopsis thaliana 20 50-200
Daucus carota
100
Lotus corniculatus
50
Nicotiana tabacum
100
Oryza sativa 20 200

* MIC: Minimal inhibitory concentration
** IC50: Concentration inhibiting growth by 50%


   
   (2) NTC – Background and mechanism of action
   


NTC is a streptothricin antibiotic produced by Streptomyces noursei. Its antibiotic effect is based on inhibition of protein biosynthesis and induction of miscoding. Resistance to NTC is conferred by bacterial sat1 or Streptomyces nat1 marker genes. The product of the marker genes - Nouresothricin N-acetyltransferase - inactivates NTC by monoacetylation of an amino group of the β-lysine residue. NTC exerts no cross-reactivity with other aminoglycosid antibiotics such as Hygromycin or Geneticin.

For more details on NTC and for ordering, please click here:

www.jenabioscience.com/cms/en/1/catalog/1114/


For references on NTC applications and further information please see

  www.jenabioscience.com/images/625337ef95/NTC_superior_selection_tool.pdf

or contact us by email at: expression@jenabioscience.com


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