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      Jena Bioscience Newsletter, Issue February 2012
   
   


* In this issue:

(1) Convenient universal site-specific fluorescent protein labeling
(2) Nourseothricin (also termed NTC or clonNAT) – the prime selection antibiotic

   
       
  
   
   

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    (1) Convenient universal site-specific fluorescent protein labeling
   


Labeling of proteins with fluorescent reporter dyes is a prominent method for their characterization with respect to their structure, folding, and interaction with other proteins both in biochemical and in cellular investigations. The novel labeling kits presented here are designed for site-specific labeling of

  • any protein's C-terminus using convenient, fast, mild, and biocompatible oxyamine-ketone ligation (Figure 1),
  • a protein's N-terminal cysteine using native chemical ligation with a fluorophore-thioester, or
  • a protein's C & N-termini simultaneously with two colors in a One-Pot Dual Color labeling reaction for fluorescence resonance energy transfer (FRET) studies

Figure 1: General method for protein modification at the C terminus: Under mild and biocompatible conditions a protein thioester is transformed with 1,2-bis(oxyamino)ethane into an oxyamino-modified species that in a second step reacts rapidly with ketones carrying a fluorescent label.
(adapted from Yi et al. (2010))

 Product
Cat.-No. Amount Price
C-terminal Protein Labeling Kit
Universal C-terminal Protein Labeling with Coumarin
FP-301-COU labeling of 5 mg protein 320 €
N-terminal Protein Labeling Kit
Universal N-terminal Protein Labeling with Coumarin
FP-302-COU labeling of 5 mg protein 380 €
Protein Dual-Labeling Kit
Universal C/N-terminal Protein Labeling with Fluorescein/Coumarin
FP-303-FLU labeling of 5 mg protein 520 €


For more information and for ordering, please visit our website at

www.jenabioscience.com/cms/en/1/catalog/1897


   
    (2) Nourseothricin (also termed NTC or clonNAT) – the prime selection antibiotic
   


Nourseothricin (also termed NTC or clonNAT) is the prime selection antibiotic for a wide variety of recombinant organisms with several thousands of published papers in the scientific literature. It is routinely used for establishing transgenic strains of Gram-positive and Gram-negative bacteria, Streptomycetes, yeast, filamentous fungi (Basidiomycota and Ascomycota), protozoa, microalgae, and plants.



  For a list of sensitive species, background and selected references
  please click here


Nourseothricin is highly stable as powder and in solution and available from small to bulk quantities. Its formulations, pack sizes and prices are available online:

www.jenabioscience.com/cms/en/1/catalog/1858



   
   

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