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* In this issue:

(1) Efficient expression of parasite proteins in LEXSY
(2) Ectopic expression of genes of interest in pathogenic Leishmania species
(3) Selected publications about LEXSY in parasitology
(4) LEXSY products – all you need for cloning, expression, strain selection, and cultivation

   
       
  
   
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    (1) Efficient expression of parasite proteins in LEXSY
   


The protein expression platform LEXSY is based on the non-pathogenic (S1-classified) protozoan Leishmania tarentolae. L. tarentolae is a close relative of all pathogenic Leishmania species as well as of other pathogens such as Trypanosomes, Plasmodium and Toxoplasma (Fig. 1).




Figure 1: Leishmania-based LEXSY is closely related to a number of the most common parasites

Due to this evolutionary proximity, the LEXSY technology is efficiently expressing parasite proteins with

  • High yields
  • Correct protein folding
  • Native post-translational modifications such as glycosylation




Figure 2: Endogenous J-binding protein (JBP1) of Leishmania was overexpressed and purified from LEXSY cells (Lane 1: host, lane 2: induced culture, lane 3: non-induced culture (Courtesy of S. Vainio, The Netherlands Cancer Institute, Amsterdam).




Figure 3: Immunoreactive Surface Proteins SAG1 and SAG2 of Toxoplasma gondii were expressed and purified from LEXSY (Lane 1: marker, lanes 3-5: SAG expression strains, lanes 2 & 6 negative controls (Courtesy of M. Ebert, FZMB, Erfurt).



   
    (2) Ectopic expression of genes of interest in pathogenic Leishmania species
   


The LEXSY vector constructs allow easy introduction of any desired gene into pathogenic Leishmania species including L. major, L. donovani, L. infantum, L. amazonensis and the trypanosomatid parasite of plants P. serpens and were used for

  • ectopic expression of host MAP kinase in L. major and L. donovani knock-out strains (courtesy of G. Spaeth, Institute Pasteur, Paris)
  • overexpression of cyclophilin in L. donovani (courtesy of G. Spaeth, Institute Pasteur, Paris)
  • expression of fluorescent proteins in pathogenic Leishmania sp. (Fig. 4)




Figure 4: Expression of fluorescent proteins in pathogenic Leishmania sp. (Courtesy of M. Yeo, London School of Hygiene & Tropical Medicine, London)



   
    (3) Selected publications about LEXSY in parasitology
   


  • Lang et al. (2005) Bioluminescent Leishmania expressing luciferase for rapid and high throughput screening of drugs acting on amastigote-harbouring macrophages and for quantitative real-time monitoring of parasitism features in living mice. Cellular Microbiology 7:383.

  • Barak et al. (2005) Differentiation of Leishmania donovani in host-free system: analysis of signal perception and response. Molecular & Biochemical Parasitology 141:99.

are available at www.jenabioscience.com/cms/en/1/browse/1119/



   
    (4) LEXSY products – all you need for cloning, expression, strain selection, and cultivation
   




Need further information? Please check out the LEXSY section at the Jena Bioscience website!

Or contact us by email at: expression@jenabioscience.com.


   
   

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