The new pLEXSY_I-2 vectors:

• represent the only eukaryotic T7-TR driven expression architecture


• permit up to 5-10 x higher expression levels as compared to first generation inducible vectors (tub integration) or constitutive vectors


• enable efficient induction of protein expression at any chosen time of cultivation


• can be used for intracellular or secretory protein expression


• offer convenient restriction sites for cloning


• allow fusion to a C-terminal affinity tag


• are available with neo or ble selection markers


• are fully compatible with constitutve LEXSY expression constructs

• Clone 5' into Bgl II, Nco I or Sla I site and get cytosolic expression


• Clone 5' into Sal I or Xba I, in frame with a signal peptide (SP), and get secretory expression


• Clone 3' into MspC I or Kpn I, in frame with His6 stretch (H6), and purify by metal affinity chromatography


• Clone 3' into Not I, and introduce your affinity tag

• LEXSY Strains & Cultivation Kits
  The LEXSY expression hosts, as strains or complete ready-to-grow cultivation kits.


• LEXSY Media, Antibiotics, Additives
  Everything for growth and maintenance of strains.


• LEXSY Molecular Tools
  Expression vectors and useful primers.


• PCR and Cloning enzymes
  All restriction enzymes for the pLEXSY-2 cloning sites, ligases, polymerases etc...

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