Amine and thiol labeling are the most common protein labeling techniques. The outstanding photostability and quantum yield of the thoroughly selected Atto-dyes combined with a high water solubility allow you to use your labeled protein in immunochemistry as well as for investigating structure, function and protein interaction.

Please refer to http://www.jenabioscience.com/cms/en/1/catalog/1179/ for information on prices and convenient labeling protocols.

Jena Bioscience has developed an innovative linker technology for attaching small fluorophores to nucleotides. The resulting conjugates are perfectly suited for enzymatic labeling of DNA by PCR and Nick Translation. Our kits are optimized for high incorporation of the fluorescent nucleotide analog as well as high product yields.

Find out more about our DNA labeling technologies at:

http://www.jenabioscience.com/cms/en/1/browse/594/

EvaGreen™ Fluorescent DNA Stain is specially developed for real-time qPCR applications including high-resolution DNA melting curve analysis. An increased stability and low interference with the PCR reaction clearly distinguishes EvaGreen™ from commonly used dyes such as SYBR® Green I.

EvaGreen™ is setting the new standard for real-time qPCR – see for yourself at:

http://www.jenabioscience.com/cms/en/1/browse/744/

Simply select the optical setting for SYBR®Green or FAM	Very clear high-resolution DNA melting curve analysis	EvaGreen™ is highly stable	Low interference with PCR
Excitation and emission spectra of EvaGreen™ is similar to SYBR® Green / FAM. Excitation max: λEx = 500 nm Emission max: λEm = 530 nm (EvaGreen™ bound to dsDNA in PBS buffer pH 7.3)	DNA melting curve analysis using EvaGreen™ and Competitor Dye with 4 different amplicons. Competitor Dye shows occasional formation of an extra melting peak.	Degeneration of SYBR® Green I within 3 hours at 99°C. EvaGreen™ shows no detectable decrease of fluorescence intensity. (Each fluorophore 1.2 µM in Tris-HCl buffer pH 9.0)	PCR amplification plots using EvaGreen™ and Competitor Dye at two different concentrations. Competitor Dye exhibits significant PCR inhibition at 1x concentration while EvaGreen™ does not.

25 years of experience in nucleotide chemistry led to linking expertise that allows attachment of fluorophores through optimized linkers to virtually any accessible position of the base, sugar or phosphate moiety of the nucleotide molecule. Their application ranges from kinetic assays in signal transduction research to single molecule sequencing approaches.^1-5

Discover our broad range of fluorescent nucleotides at:

http://www.jenabioscience.com/cms/en/1/browse/680/

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