Functionalization of DNA is efficiently accomplished by PCR amplification involving unnatural nucleoside triphosphates carrying the desired function. EdUTP (5-Ethynyl-dUTP) can be used to generate large and highly modified DNA strands by PCR incorporation, replacing the natural substrate dTTP. The resulting densely alkyne-modified DNA can be subsequently derivatized by "click" reactions (Figure 2), such as Cu(I)-catalyzed azide-alkyne cycloadditions,,.
Chemical structure of EdUTP (Cat.-No.: CLK-T07)
Figure 2: "Click reaction" between alkyne-modified DNA and different azides
EdUTP and more click chemistry reagents can be found at: www.jenabioscience.com/cms/en/1/catalog/1359/
Nucleotide analogs not on stock may be available as custom synthesis, just send us an e-mail at firstname.lastname@example.org with any questions you may have!
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