Jena Bioscience - Newsletter

NCoR and SMRT are well-known corepressors crucial for the assembly of functional holocomplexes that lead to gene silencing [1-3]. Listen to the sound of silence with our well-established NCoR and SMRT antibodies.

Due to their pivotal role in histone modification and chromatin remodeling, NCoR and SMRT have gained tremendous importance in the field of Epigenetics in recent years [4]. They are well-known corepressors of nuclear receptors but interactions have been shown with various protein families:

Our NCoR and SMRT antibodies are convenient tools for studying corepressor complexes and the corresponding pathways. They are suitable for Western Blot analyses, Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP) and Immunohistochemistry [5]. Anti-NCoR has been successfully applied in precipitation of HDACs e.g. for HDAC activity assays [6].

References:
[1] Rosenfeld et al. (2006) Sensors and signals: a coactivator / corepressor / epigenetic code for integrating signal-dependent programs of transcriptional response. Genes Dev. 20:1405.
[2] Heinzel et al. (1997) A complex containing N-CoR, mSln3 and histone deacetylase mediates transcriptional repression. Nature 387:43.
[3] Nagy et al. (1997) Nuclear receptor repression mediated by a complex containing SMRT, mSin3A, and histone deacetylase. Cell 89:373.
[4] Perissi et al. (2010) Deconstructing repression: evolving models of co-repressor action. Nat. Rev. Genet. 11 (2):109.
[5] Tiefenbach et al. (2006) SUMOylation of the Corepressor N-CoR Modulates Its Capacity to Repress Transcription. Mol. Biol. Cell. 17:1643.
[6] Göttlicher et al. (2001) Valproic acid defines a novel class of HDAC inhibitors inducing differentiation of transformed cells. EMBO J. 20 (24):6969.