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   Extended range of Click Chemistry Reagents from Jena Bioscience
   
   

(1) Alkyne-containing phosphoramidites
(2) Propargyl-CPGs
(3) Alkyne-, DBCO-, and Azide-reagents

   
       
  
   
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Click Chemistry has become an efficient and widely used labeling technique. We are happy to announce that our portfolio of Click Chemistry reagents has been extended by numerous Alkyne-containing Phosphoramidites and 2'-O- and 3'-O-Propargyl-CPGs (Controlled Pore Glasses) for synthesis of oligonucleotides by automated standard protocols.

The resulting oligos contain reactive groups (triple bonds) for subsequent CLICK-attachment of reporter groups such as biotin or fluorescent dyes, allowing synthesis of highly modified DNA strands carrying multiple labels in a density unmatched by classic labeling techniques[1]. The labeled oligos may serve as PCR primers[2], antisense agents for therapeutic applications[3],[4], molecular beacons for detecting genetic mutations, and as probes for measuring gene expression in DNA microarrays[5] and gene chips.


   
   Alkyne-containing Phosphoramidites
   
Figure 1: Molecular structure of 2'-O-Propargyl-3'-CEP Adenosine (Bz) [Cat.No.: CLK-ANP-7751]


We offer a large selection of phosphoramidites [e.g. 2'-O-Propargyl-3'-CEP Adenosine (Bz), Fig. 1] for synthesis of oligonucleotides by automated standard protocols, including:

  • 2'-O-Propargyl / 3'-O-Propargyl-CEPs of A, C, G, U, I
  • 5-Alkynylated-CEPs of dU / dC



For more detailed information please click here:

www.jenabioscience.com/cms/en/1/catalog/1380


   
    2'-O- and 3'-O-Propargyl-CPGs
   


In addition, we offer a large variety of CPGs (Controlled Pore Glasses) as supports for the synthesis of oligonucleotides by automated standard protocols, including:

  • 2'-O-Propargyl / 3'-O-Propargyl-CPGs of A, C, G, U, I - available with 500 Å or 1000 Å pore sizes either as bulk material or in pre-filled columns



For more detailed information please click here:

www.jenabioscience.com/cms/en/1/catalog/1612


   
    Jena Bioscience's product line of Click Chemistry Reagents also contains:
   


Click Chemistry Kits
 
Other Alkyne-containing CPGs
 
Alkyne-containing Nucleosides
 
Alkyne-containing Spacers
 
Alkyne-containing Nucleotides
 
Alkyne-containing Fluorescent Dyes
 
Alkyne-containing Phosphoramidites
 
Auxiliary Reagents
 
2'-O- and 3'-O-Propargyl CPGs
 

 




DBCO-containing Chemical Modification Reagents
 
DBCO-containing Spacers
 
DBCO-containing Biotinylation Reagents
 
DBCO-containing Fluorescent Dyes
 




Non-Fluorescent Azides
 
Azides of Fluorescent Dyes
 
Biotin Azides
 
Azides of Quencher Dyes
 
PEG and Spacer Azides
 
Nucleotide Azides
 
Azides of Amino Acids
 

 


Selected References:
[1] Kiviniemi et al. (2010) Solid-supported synthesis and click conjugation of 4'-C-alkyne functionalized oligodeoxyribonucleotides. Bioconjugate Chem. 21:1890.
[2] Ju et al. (2003) Click chemistry to construct fluorescent oligonucleotides for DNA sequencing. J. Org. Chem. 68:609.
[3] Ma et al. (2000) Oligonucleotides as therapeutics: the coming of age. Biotechnol. Annu. Rev. 5:155.
[4] Herdewijn (2005) Methods in Molecular Biology, Ed. Oligonucleotide Synthesis: Methods and Applications; Human Press Inc: Totowa 288.
[5] Collman et al. (2005) Chemoselective covalent coupling of oligonucleotide probes to self-assembled monolayers. J. Am. Chem Soc. 127:8600.


   
   

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Germany

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