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Click Chemistry – fast, mild & reliable DNA labeling

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Click chemistry is a chemical philosophy introduced by K. Barry Sharpless in 2001 [1] and describes chemical reactions that generate substances quickly, reliably and in quantitative yield by joining small building blocks under mild conditions. This is inspired by the fact that chemical reactions in nature also generate substances by joining small modular units.

One of the most popular reactions within the click chemistry philosophy is the so called azide-alkyne Huisgen cycloaddition, using a Copper (Cu) catalyst at room temperature.

Click chemistry reactions allow a DNA labeling density unreached by "classic" labeling techniques [2,3]. Click chemistry has also been applied for ultra-sensitive detection methods such as DNA photography [4]. For even more application examples, please see [5].

Now available from Jena Bioscience:

A complete range of Click Chemistry Reagents

Phosphoramidites
Phosphoramidites for the synthesis of oligonucleotides containing reactive triple bonds, allowing attachment of reporter groups by click chemistry techniques

Nucleotides
Nucleotides used to generate DNA containing reactive triple bonds, allowing attachment of reporter groups by click chemistry techniques

Non-Fluorescent Azides
Azides for click chemistry attachment of non-fluorescent reporter groups to DNA containing reactive triple bonds

Azides of Fluorescent Dyes
Azides of fluorescent dyes for click chemistry attachment to DNA containing reactive triple bonds

PEG and Linker Azides
Azides for PEGylation of DNA and for incorporation of linkers by click chemistry techniques

Auxiliary Reagents
Catalysts, ligands and solvents for click reactions

For a complete overview of our Click Chemistry Reagents and for background information, please visit our website at

www.jenabioscience.com/cms/en/1/browse/1379/

Selected References:
[1] Kolb et al. (2001) Click chemistry: diverse chemical function from a few good reactions. Angew. Chem. Int. Ed. 40 (11):2004.
[2] Gierlich et al. (2007) Synthesis of Highly Modified DNA by a Combination of PCR with Alkyne-Bearing Triphosphates and Click Chemistry. Chem. Eur. J. 13:9486.
[3] Gramlich et al. (2008) Click-Click-Click: Single to Triple Modification of DNA. Angew. Chem. Int. Ed. 47:3442.
[4] Hammond et al. (2007) DNA Photography: An Ultrasensitive DNA-Detection Method Basedon Photographic Techniques. Angew. Chem. Int. Ed. 46:4184.
[5] for a comprehensive list of click-chemistry related publications, please see www.scripps.edu/chem/sharpless/click.html

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