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   Cell Penetrating Peptides: Internalization of proteins into live cells
   

Since the first discovery of Cell Penetrating Peptides (CPPs) some 20 years ago quite a number of peptides were found to be able to transport different cargos (DNA, RNA, peptides, proteins and nanoparticles) into a variety of live cells in vitro and in vivo. Since transport of cargo was demonstrated e.g. for human stem and nerve cells as well as through the blood-brain and skin barrier CPPs recently gained significant attention for diagnosis and treatment of diseases.

Jena Bioscience offers a selection of the most prominent CPPs (see table below) ready to use for simple mixing with cargo for direct internalization. In addition, the multi-component cocktail JBS-Proteoducin was designed containing a balanced mixture of several CPPs and auxiliary compounds optimized for efficient internalization of proteins such as enzymes and antibodies (Table 1). All our CPPs

  • are applicable to many cell lines including both adherent and suspension cells
  • have low toxicity for live cells
  • are targeted to different internalization mechanisms thereby achieving higher success rates
  • are able to internalize antibodies which usually act more specific than enzyme inhibitors and are able to compete with silencing RNA techniques
  • are able to enrich intracellular concentrations of cargos in the low µmole range
  • allow straight forward experiments, no expensive equipment necessary
  • can be used with selected helper reagents for fine-tuning of experimental set ups
  • are safe to use without any special precautions

Please note: Cells show different sensitivity to high concentrations of CPPs, cargos and auxiliaries and therefore should be tested for viability and membrane integrity. Multicomponent cocktails can trigger different uptake mechanisms and are thus less specific for cells and cargos. They may therefore serve as a starting point for internalization studies however, we recommend the use of single peptides when optimizing a particular internalization problem.

CAD-2, MPGα and MPGβ show different chemical properties, e.g. polarity, amphiphilicity and stability against degradation by membrane bound and secreted proteases. Therefore, peptide selection highly depends on the cells and membranes used in a particular experiment. CAD-2 is the most hydrophobic peptide, exhibits the highest stability against proteolytic degradation and shows the highest uptake efficiency.

 Product
Cat.-No. Amount Price
 Complete Internalization Kit
 Transduction Kit for Peptides and Proteins  (Proteoducin-based)
CPP-K01
25 ... 200 reactions
300 €
 Internalization Cocktail
 JBS-Proteoducin
CPP-C01S
250 reactions
90 €
CPP-C01L
1250 reactions
360 €
 Cell Penetrating Peptides
 MPGα
CPP-P03S
0.5 mg
90 €
CPP-P03L
2.5 mg
360 €
 MPGβ
CPP-P04S
0.5 mg
90 €
CPP-P04L
2.5 mg
360 €
 CAD-2
CPP-P05S
0.5 mg
90 €
CPP-P05L
2.5 mg
360 €
 CPPP-2
CPP-P06S
1.2 mg
25 €
CPP-P06L
6 mg
100 €
 Cell Integrity Kits
 MTT Cell Viability Assay
CPP-K03
25 assays
132 €
 Mycoplasma Detection Kit
PP-401S
10 reactions
80 €
PP-401L
50 reactions
180 €
 Auxiliary Compounds
 
Streamlined selection of reagents that help controlling and optimizing cell transfection
 

Please find more details about our Cell Penetrating Peptides, incubation procedures and internalization efficiencies, at our website, in our General Manual and in our publications:


Reference:
[1] Mussbach et al. (2011) Transduction of peptides and proteins into live cells by cell penetrating peptides. J. Cell. Biochem. 112:3824.
[2] Mussbach et al. (2011) Internalization of nucleoside phosphates into live cells by complex formation with different CPPs and JBS-Nucleoducin. Methods in Molecular Biology 683:375.

   
   

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