Jena Bioscience - Newsletter

Affinity Chromatography Media and Kits from Jena Bioscience

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Immobilized nucleotides provide a convenient and rapid one-step purification procedure for a large number of proteins such as kinases, GTPases, chaperones, motor proteins, and others. Jena Bioscience affinity chromatography material is tailor-made for purification (or for related assays) of particular NTP-binding proteins since we provide:

NTPs and dNTPs that are linked to the matrix at various positions of sugar, base or phosphate moiety of the nucleotide
different types and lengths of linkers
different types of chromatography material ranging from bulk material to pre-made columns that fit any machine

The following is a selection of immobilized nucleotides that have recently been reported in the literature:

γ-Aminophenyl-ATP-Sepharose^®-C₁₀-Spacer (JBS Cat. No.: AC-101) more...

Vaccaro et al. (2006) A rapid ATP affinity-based purification for the human non-receptor tyrosine kinase c-Src. Protein Expression and Purification 46:240.

Application:	Rapid and simplified purification of ΔN85 c-Src protein from recombinant baculovirus-infected High 5 insect cells

γ-Aminophenyl-ATP-Sepharose^® (JBS Cat. No.: AC-102) more...

Mlakar and Legiša (2006) Citrate inhibition-resistant form of 6-Phosphofructo-1-kinase from Aspergillus niger. Appl. Environ. Microbiol. 72 (7):4515.

Application:	Purification of phosphofructo-1-kinases (PFK1) from Aspergillus niger

N⁶-Aminohexyl-ATP-Sepharose^® (JBS Cat. No.: AC-129) more...

Bendz et al. (2007) Human heat shock protein 70 enhances tumor antigen presentation through complex formation and intracellular antigen delivery without innate immune signaling. J. Biol. Chem. 282 (43):31688.

Application:	Purification of recombinant human Hsp70 protein

γ-Aminohexyl-GTP-Sepharose^® (JBS Cat. No.: AC-117) more...

Dawson et al. (2007) Parkinson's disease-associated mutations in LRRK2 link enhanced GTP-binding and kinase activities to neuronal toxicity. Human Molecular Genetics 16 (2):223.

Application:	GTP/GDP-binding studies on leucine-rich repeat kinase 2 (LRRK2) variants

m⁷-GTP-Sepharose^® (JBS Cat. No.: AC-141 / AC-142) more...

Miyoshi et al. (2006) Binding analyses for the interaction between plant virus genome-linked protein (VPg) and plant translational initiation factors. Biochimie 88:329.

Application:	Investigation of the interactions between genome-linked protein (VPg) of the Potyvirus TuMV, and A. thaliana initiation factors by using affinity chromatography

ATP Affinity Test Kit (JBS Cat. No. AK-102)

Screen your crude protein solution for the most suitable chromatographic material!

The ATP Affinity Test Kit provides a quick and easy way to determine the most suitable ATP-Sepharose^® derivative for the purification of ATP-binding proteins. The kit contains everything for screening a crude protein solution for the most suitable chromatographic material.

We have even more affinity material in stock! Please check out our sections:

To browse our whole portfolio of Affinity Chromatography products visit:

http://www.jenabioscience.com/cms/en/1/browse/802/

If you require affinity chromatography materials that are not on stock please send us an e-mail at info@jenabioscience.com to receive a quote for a custom synthesis!

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Jena Bioscience GmbH
Loebstedter Str. 80
07749 Jena
Germany

Phone:+49 – 3641 – 6285 000
Fax: +49 – 3641 – 6285 100
E-Mail: info@jenabioscience.com

Register Court: Amtsgericht Jena, HRB 207171
VAT No.: DE 195825742

Managing Directors:
Thomas Billert
Dr. Mathias Grün

www.jenabioscience.com