6 % CL-Nickel ChroMatrix™ is a superior, 6 % cross-linked IMAC resin that uses nickel ions for purifying recombinant polyhistidine (His-) tagged proteins. Its unique properties facilitate the rapid and high-yielding one-step purification from crude lysates both under denaturing and non-denaturing conditions.
- Suitable for FPLC, gravity flow and batch purification
- Excellent binding capacity based on high surface area to volume ratio
- Fast flow rates
- Notable mechanical and chemical stability
- Reusable without decrease in yield
Nickel ChroMatrix™, containing the iminodiacetic acid (IDA) chelating group, has been successfully used in a one-step purification of recombinant pyruvate kinase from a crude cell lysate:
M = Molecular weight marker, FT = Flow through, W1-W2 = Wash 1-2, E1-E4 = Elution 1-4
SDS PAGE analysis of recombinant pyruvate kinase (PK) purification:
His-tagged PK was expressed in E. coli, purified by one-step chromatography with 6 % CL-Nickel ChroMatrix™ and subsequently analyzed by SDS-PAGE.
|6% CL Nickel ChroMatrix™
For Cobalt ChroMatrix™ please refer to: www.jenabioscience.com/cms/en/1/catalog/1467/
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 Porath et al. (1975) Metal chelate affinity chromatography, a new approach to protein fractionation. Nature 258:598.
 Gaberc-Porekar et al. (2001) Perspectives of immobilized-metal affinity chromatography. J. Biochem. Biophys. Methods 49:335.
 Ueda et al. (2003) Current and prospective applications of metal ion-protein binding. Journal of Chromatography 988:1.