IMAC – Immobilized Metal Affinity Chromatography


Please click here if this newsletter is not displayed properly!

Important note: If you have questions, comments or want to get in contact with us directly, please DO NOT reply to this newsletter. Please send your inquiry to info@jenabioscience.com.


Immobilized metal affinity chromatography (IMAC) is most frequently used for purification of polyhistidine (His-) tagged proteins. The technique is based on the interaction between exposed protein surface residues (preferentially histidines) with transition metal cations (Cu2+, Ni2+, Zn2+, Co2+). The transition metal itself is immobilized to a cross-linked agarose matrix via a chelating group such as iminodiacetic acid (IDA)[1].

Based on the HSAB concept, IDA-immobilized Cu2+, Ni2+, Zn2+ and Co2+ ions exhibit varying affinities & specificities toward histidines[2,3]. While Ni2+ ions show a high affinity but low specificity, Co2+-ions are more specific but with reduced binding affinity and are therefore, an option to reduce non-specific protein binding.

Jena Bioscience offers a wide range of reagents for immobilized metal affinity chromatography including:

    Nickel ChroMatrix™, Fast Flow

6 % CL-Nickel ChroMatrix™ is a superior, 6 % cross-linked IMAC resin that uses nickel ions for purifying recombinant polyhistidine (His-) tagged proteins. Its unique properties facilitate the rapid and high-yielding one-step purification from crude lysates both under denaturing and non-denaturing conditions.


  • Suitable for FPLC, gravity flow and batch purification
  • Excellent binding capacity based on high surface area to volume ratio
  • Fast flow rates
  • Notable mechanical and chemical stability
  • Reusable without decrease in yield

Nickel ChroMatrix™, containing the iminodiacetic acid (IDA) chelating group, has been successfully used in a one-step purification of recombinant pyruvate kinase from a crude cell lysate:

M = Molecular weight marker, FT = Flow through, W1-W2 = Wash 1-2, E1-E4 = Elution 1-4

SDS PAGE analysis of recombinant pyruvate kinase (PK) purification:

His-tagged PK was expressed in E. coli, purified by one-step chromatography with 6 % CL-Nickel ChroMatrix™ and subsequently analyzed by SDS-PAGE.

Product Cat.-No. Amount Price
6% CL Nickel ChroMatrix™

Bulk material
AC-202-10 10 ml 63,00 €
AC-202-50 50 ml 252,00 €
AC-202-100 100 ml 483,00 €
AC-202-500 500 ml 2.362,50 €

For Cobalt ChroMatrix™ please refer to: www.jenabioscience.com/cms/en/1/catalog/1467/

  Download our Affinity Chromatography Mini Catalog in PDF format (2.9 MB) and get an overview of our entire Affinity Chromatography product portfolio!

Selected references:
[1] Porath et al. (1975) Metal chelate affinity chromatography, a new approach to protein fractionation. Nature 258:598.
[2] Gaberc-Porekar et al. (2001) Perspectives of immobilized-metal affinity chromatography. J. Biochem. Biophys. Methods 49:335.
[3] Ueda et al. (2003) Current and prospective applications of metal ion-protein binding. Journal of Chromatography 988:1.


If you wish not to receive further email newsletters from us, please reply to this E-mail with the subject "UNSUBSCRIBE" or unsubscribe via the form at our website at www.jenabioscience.com.

   Responsible for content / Imprint
Jena Bioscience GmbH
Loebstedter Str. 80
07749 Jena

Phone:+49 – 3641 – 6285 000
Fax: +49 – 3641 – 6285 100
E-Mail: info@jenabioscience.com

Register Court: Amtsgericht Jena, HRB 207171
VAT No.: DE 195825742

Managing Directors:
Thomas Billert
Dr. Mathias Grün
      © 2011 Jena Bioscience GmbH - Imprint