Jena Bioscience introduces a new system for quantitative real-time PCR based on the fluorescent DNA stain EvaGreen™. This green fluorescent dye enables rapid analysis of any target DNA without additional sequence-specific DNA probes. EvaGreen™ is compatible with all common Real-Time PCR cyclers - simply select the standard settings for SYBR^® Green or FAM!

EvaGreen™ Fluorescent DNA Stain is a superior intercalator dye specially developed for real-time qPCR applications including high-resolution DNA melting curve analysis. EvaGreen™ is highly stable both thermally and hydrolytically, resulting in minimal quenching. Combined with its high quantum yield and low interference with PCR it is the ideal fluorophore for real-time PCR and a superior and convenient replacement for commonly used dyes such as SYBR^® Green I.

Simply select the optical setting for SYBR®Green or FAM	Very clear high-resolution DNA melting curve analysis	EvaGreen™ is highly stable	Low interference with PCR
Excitation and emission spectra of EvaGreen™ is similar to SYBR® Green / FAM. Excitation max: λEx = 500 nm Emission max: λEm = 530 nm (EvaGreen™ bound to dsDNA in PBS buffer pH 7.3)	DNA melting curve analysis using EvaGreen™ and Competitor Dye with 4 different amplicons. Competitor Dye shows occasional formation of an extra melting peak.	Degeneration of SYBR® Green I within 3 hours at 99°C. EvaGreen™ shows no detectable decrease of fluorescence intensity. (Each fluorophore 1.2 µM in Tris-HCl buffer pH 9.0)	PCR amplification plots using EvaGreen™ and Competitor Dye at two different concentrations. Competitor Dye exhibits significant PCR inhibition at 1x concentration while EvaGreen™ does not.

Our EvaGreen™ qPCR product series provides easy-to-handle and powerful tools for quantification of sample DNA in a broad dynamic range of up to 6 orders of magnitude with exceptional sensitivity and precision. The high specificity and sensitivity of our EvaGreen™ reagents is complemented by an optimized hot-start polymerase preventing extension of non-specifically annealed primers or primer-dimer formations at low temperatures during PCR setup.

Our entire product line is designed to save you valuable time at the bench and yielding superior results and performance. All materials are accompanied by a streamlined and easy to follow documentation. In addition, check out the prices, and you may discover that there is a chance of cutting your annual reagents spending by some 50%...!

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