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   New: Kits and single reagents for DNA labeling
   
       
       
  
   
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Jena Bioscience is now offering a product line for direct enzymatic incorporation of fluorescent labeled nucleotides into DNA. The thoroughly composed kits are suitable for all typical DNA labeling applications such as FISH, microarray gene expression profiling, single molecule detection and other nucleic acid hybridization assays.

The Kits contain all reagents required for DNA labeling providing a highly efficient, easy-to-perform and rapid labeling procedure. The excellent stability and quantum yield of the fluorophores combined with a high incorporation rate of the dye-dUTP complex makes it the ideal choice for a broad range of fluorescence applications.

Please visit our complete section for DNA labeling products at: http://www.jenabioscience.com/index.php//1/browse/594



Our proprietary linker technology provides you with fluorescent labeled dUTP analogs for incorporation into DNA at highest rates.
Whole chromosome painting of chromosome 21. Fluorescent labeling of WCP21 by nick translation using Atto550 NT Labeling Kit.

   
   

Fluorescent labeling by PCR

In this PCR reaction dTTP is partially substituted by dye-dUTP. The Taq polymerase efficiently incorporates these dye-dUTPs resulting in fluorescent labeled double-stranded DNA.

Emission Color Product
(Labeling Kit / Dye-Nucleotide)
Absorption max. [nm] Emission max. [nm] Replacement for
blue-green Atto425 PCR Labeling Kit

Atto425-dUTP-PCR
436 484 DEAC
green Atto488 PCR Labeling Kit

Atto488-dUTP-PCR
501 523 Alexa Fluor 488, Fluorescein (FITC), FAM, Oregon Green 514, Rhodamine green, Rhodamine 123
yellow Atto550 PCR Labeling Kit

Atto550-dUTP-PCR
554 576 Alexa Fluor 555, Cy 3, Alexa Fluor 546, TAMRA, Rhodamine Red, Spectrum Orange
orange TexasRed PCR Labeling Kit

TexasRed-dUTP-PCR
583 603 Rhodamine ITC, Cy 3.5, ROX, Alexa Fluor 568
orange Atto590 PCR Labeling Kit

Atto590-dUTP-PCR
594 624 Alexa Fluor 594, Alexa Fluor 610
red Atto647N PCR Labeling Kit

Atto647N-dUTP-PCR
644 669 Alexa Fluor 647, Cy 5
far-red Atto655 PCR Labeling Kit

Atto655-dUTP-PCR
663 684 Alexa Fluor 660, Cy 5.5

   
   

Fluorescent labeling by Nick Translation

Nick translation labeling based on the reverse activities of DNA polymerase I and DNase I is used to randomly replace dTTP with dye-labeled dUTP in DNA probes. The well balanced activities of the enzyme mix ensure the generation of highly labeled double stranded DNA fragments.

Emission Color Product
(Labeling Kit / Dye-Nucleotide)
Absorption max. [nm] Emission max. [nm] Common Filter Set Alternative Fluorophore
blue-green Atto425 NT Labeling Kit

Atto425-dUTP-NT
436 484 DEAC DEAC
green Atto488 NT Labeling Kit

Atto488-dUTP-NT
501 523 Fluorescein Alexa Fluor 488, Fluorescein (FITC), FAM, Oregon Green 514, Rhodamine green, Rhodamine 123
yellow Atto550 NT Labeling Kit

Atto550-dUTP-NT
554 576 Cy 3
Spectrum Orange
Alexa Fluor 555, Cy 3, Alexa Fluor 546, TAMRA, Rhodamine Red, Spectrum Orange
orange Atto590 NT Labeling Kit

Atto590-dUTP-NT
594 624 Texas Red Texas Red, Alexa Fluor 594, Alexa Fluor 610

   
   

Non-fluorescent Labeling by PCR

Label Product
(Labeled Nucleotide)
Molecular Weight
Biotin Biotin-XX-aa-dUTP 972.81(Anion)


Our entire product line is designed to save you valuable time at the bench and yielding superior results and performance. All materials are accompanied by a streamlined documentation that maximizes success. In addition, check out the prices, and you may discover that there is a chance of cutting your annual reagents spending by some 50%...

Please contact us for inquiries and further information or request your customized quotation.


   
   

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Jena Bioscience GmbH
Loebstedter Str. 80
07749 Jena
Germany

Phone:+49 – 3641 – 6285 000
Fax: +49 – 3641 – 6285 100
E-Mail: info@jenabioscience.com

Register Court: Amtsgericht Jena, HRB 207171
VAT No.: DE 195825742

Managing Directors:
Thomas Billert
Dr. Mathias Grün
   
           
    http://www.jenabioscience.com   
           
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