pLEXSY_I-egfp-ble3     EGE-247

Description:

nt 0001-0741:		5īpart of odc gene of Leishmania tarentolae for chromosomal integration
nt 0742-0758:		T7 promoter
nt 0768-0784:		TET operator
nt 0820-1176:		5īnon-translated region of aprt gene of Leishmania tarentolae 
			with splice acceptor site for target gene
nt 1186-1905: 		egfp gene
nt 1915-3254:		1.4k intergenic region from cam operon of Leishmania tarentolae 
			with polyA site for target gene and splice acceptor site for marker gene 
nt 3266-3640:		ble marker gene
nt 3681-4258:		5'UTR of dhfr-ts gene of Leishmania with polyA site for marker gene
nt 4382-5164:		3īpart of odc gene of Leishmania tarentolae for chromosomal integration
nt 5166-7116:		pUC18 derived with ori and bla marker gene @ nt 5449-6309.


ATTTAAATGAATACTTACAAGGTACTCCTGCACCCGGCACAAGCGTATGTAATTGCTAGCACGCCGGTGCCGACCCAACCTCCAACTCCCGTGCTCACGCGCGCACACACGCACGCACAAGCATCATCATCATCATCATCATGGTCATCCCTCCCTTATCTGGCGAAGGAGGCACTGTGACGCAAGGTTTTCTGTGTCTCTCCTACCCCTCTACTGATTCCTTCTTTCTCTCTTCCTCTTCTCGCACTGCACCCCCCTCCCCCCCCCCACACACACACACTTGCTGTCATGGCTGTGCGCATTCGCTCACTCTTGTCGTGTCCCGTTTCCCTCATGGCGTCGTCGTCTTCCCCTGCGCAACCACCCACGCTGCACACGCACGCTCCACAGCGCGCTTCGCCTTCTTCGCTCTCTCCCTAGCCTCCTCTCTGTCACCCTCCACTGCCGCCGCGGTTGGCCGCTCTGCATCCTTTTCCCGCAGCCTTTCCTTTTTTTTTTCACTGTCAGTCTGGCTCATACCCGTGGAGCTGTGTCTCCCCGAGCTTCCCATACACCCACCCCCCACGACCTTTCTCAGCTTCCTTTCTCAGTCGCTTGAGCACGCAGTGCATTCACTTCTTCGTCCCTCAGTTCGCCTCGCTCTCGCTCTCTCTCGCGCGCGCTGCCGTACCCGCCCCTCCTCGTACTCCCCTCCTTCCTCATCCCCACCCCCCCCGTGTGCGCCGGCTCCTCTCTGAATTCTAATACGACTCACTATAGGGAGATCGTCCCTATCAGTGATAGAGATCCAGTCGCAGCCTGACCGCATCACACATCAAGGCGTTACAGCCTCTCCGTCTTTGCTGCACCCAAGTGTATTCGTGTGACCGCGTTGAGGGATCTAATGGATTCGGAACTTTGGTCGTTTGGTCTCTCATGCTGCCCAATCCCATGCGCTTTTCTCCGTGCCTCTCTGTCTCCCCTTCCGTTCTATTTGGACAACGATGTGTACTGTAATGCGTGCGCCATCTGACAACGAATAGATCAGCAGCATTCGCACACTTGCACATACCCAGTGAAGCTTTTGTGTCTGTCGTATTGACAACACCGACTGCAACAAGGTGTAGATAGAAGTTGGCCTTCTCGCTCGCTCGCACGCTCTTCACGCTCCTGCTTTCCTTGCTGTGCCTTGCCACCAGATCTGCCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAAAGCGGCCGCCCTCCTCCTCCTTTCTTGTTCCTTTCACGTCGCCTTCTCGGTTGTAGCTGGCAGACGACGAGTCTTACTTTTACGTGTACTTCTCTATAGATGATGTATGATCTCTCTGCATGCGTGTTCGTGCATGTGTCCGTGTGTTGTGTACGCGTGCGTCTCGCCTCAGCTCTCCGCGTGAAAGGGTTTGACTGCCCATGATGCGTGTGTATATCCACGCGCAGGCACGCACACACACACACACACACACACACACAGGCACACACAGGCACACAAACGCATCTCAGGCCGAGCCGCATACGTCTCTCGCACGGTCTCGTTTATTTGATCATGTAGTTGAGTATTAAATTGGGAAGACAAAAACATAATAGCACGAAGAGTCGGGCACGAAAAGCCCGATCTCTCTCTCTCTCTCTCGTGCGCGCAGGGGCGTGTGGGTGCACGACGACGAGGACGGAGGGGGGAAGGGAGGCATAAACGGATCGATCCCCCATCGCACATGCGGGTACGAGCATTATCTGCTGTGTCTGGTCCTTTATCATATCGCTACCCGCCCGCCCCCCGCCCCCCTCCCCCCCCCCCCCCCCGCGGCTCCTGTCACTGTCGTTCCTGCGACTCCCCACACACCCGCGCAACGCTCATGTCACGAAGAGAAAAGTAGAAGGCGTGGCGATGCGTTGCGCGGCCCCCTGTCCGCTGGCATGCAGACGTCGAGCCGTGGAACGGATGGTAGCGAGAGACAACGCGGCGATGCAGGAAAGGAGATTTACTGCAGGACGTCTACACACGCGCGCACACCACTGCGAATGGCACCGTGCTGAGGAAAATTGGGGGGGAGGGCGCACGGGGGCGGGGCAGGAAACGGTTGGATCAGCAGCACTCTCAACTTGTGTCCGTATTGCGAAGGAGGGAGAGGAGCCTGCCCGTATTATGGGCGCTGAAACGTCGGAACCACCTATGAATCCCACTTCTGCGTCGCGGAGGCGTACTGCTTCCGACCACCTCCGCAGCTCACGCGCGTCGAGCTCCCTCCCTTTCCCCTTCTTTCCGTTGTGTGTGTGTGTGTGTGTGTGTCTTGTCACGATGCATGGCCATCTCTCCTCCAACCCTCCACGCCTTCCTCTCCCCCGCCCATCAAACGCGCTACGGCCACAACCATTTCTTCAAGTATCACATCCACCACCACTCTTACCCACCTACCCTCTTCGACTCTGACACAGCCACTGACGCCCTCTCCGCCTCTCTCGCTCGCTGCACATTGACTCTCCACAGCCCCCCCTCCCTCGCACAGCCAGCCCCACCCACCCACCCACCCACCCACCGCTCTACACACCTCCACAGCAGCCGGATCCACCGCATGGCCAAGTTGACCAGTGCCGTTCCGGTGCTCACCGCGCGCGACGTCGCCGGAGCGGTCGAGTTCTGGACCGACCGGCTCGGGTTCTCCCGGGACTTCGTGGAGGACGACTTCGCCGGTGTGGTCCGGGACGACGTGACCCTGTTCATCAGCGCGGTCCAGGACCAGGTGGTGCCGGACAACACCCTGGCCTGGGTGTGGGTGCGCGGCCTGGACGAGCTGTACGCCGAGTGGTCGGAGGTCGTGTCCACGAACTTCCGGGACGCCTCCGGGCCGGCCATGACCGAGATCGGCGAGCAGCCGTGGGGGCGGGAGTTCGCCCTGCGCGACCCGGCCGGCAACTGCGTGCACTTCGTGGCCGAGGAGCAGGACTGACCGACTAGTTCTAGTTCTAGGGGCCGAATTAATTCAGATCCTCGTGTGAGCGTTCGCGGAATCGGTCGCTCGTGTTTATGCCCGTCTTGGTGTTGTGCTCGCAAGGCGGTGCAGCAGGATACCGTCGCCCTCCTCTCTCCTTGCTTCTCTGTTCTTCAATTCGCGATCTCACAGAGGCCGGCTGTGCACGCCCTTCCTCACCCTCCTTTTCCCACCTCTCGGCCACCGGTCGGCTCCGTTCCGTCTGCCGTCGAGAAGGGACGGGCATGTGCAGCTCCTCCCTTTCTCTCGCGCGCGCATCTTCTCTTGCTTGTGGCACTCACGCTCATGCGTCAAGGCGGCCCCACGCCGAGCCCCTGCGCTCCCTTCCCTCTTGCGCATCCGTAGCGCGGATGCCGTCGATGCGCAAGGCCGGCATGAAGGAGCGCGTGCCCTCAAGAGGGCACACTATCATGCCCTACGTGGGCCACGCAGCGATGAGGCCGGCTTCGGCGGAGATGCGTCACGCACGTGCCAGATGATGCGCTACGCCTTCCTTGACTTGCGCCCCCCTCTCTTCCTCCGTCTCTCACTCTCTCTCTCTCACACACACACACACACACACACACACACAAAGCTCCGGTTCGTCCGGGCCCTGCAGGCATGCCGACGTTCGGTGACCTGAACCACCTCGTCGCCGCTGTGATGTCTGGCGTGACCTGCTGCCTGCGCTTCCCTGGCCAGCTGAACTCTGACCTGCGCAAGCTTCTCCTGCTCAACGACGGCGACGGCGCTGACGAGGTGCAGAGTGGCACAGAAGCCGCAGCGGCGTACAGCGACGAGGAGGGCGGGAAGTCGCCGAGTGGTCCCCTGGCGAACGACTCCCTGTTCATGTCCGCGTGGGACCGCCGTCGCAACCTCACGAGCCGCCCGCTGCGCATCACAACCATATTCGGCCCCACCTGCGATTCGATGGACTGCATTCTGAAGAAGCAGCCCTTCCCGGAGATGAAGCTCGGCGACTGGCTTCTAGTGCCAGACATGGGCAGCTACACCACCGCTGCCGCGGGAGTCTTCAACGGTTTCGCGACACGCCGCCTTGAGTTTGTGAGCTCTGTTGACTTGTCTGCGAGGTCGAGGGCTGTGCATGCGCATGAGGGCAACACTTTTGAGTTCGTGAGCGAGTGAAAACGGGGCTGAGAGGATACAAGGTGCTGTAGGATGAGATCCTCCGCCCACCAGAGCATGGGCTATCTCTTGTGTCTGTTCTGAGCCTGCCTCTCTCTCTCTCTCTTGCCTCCCCCTCCCCCCTCCCCACACACACCCCACACGCACGCTCTGCGTCACCCTCTCCTCCCTCAGTAACGGGAGTAGACATGCAGAAGAAGTATACCACTAATACGTATGCGTTCCTGCAGGCATCCGAGATGGACACCAGAGCAAGCGCGGGTGTGCGCGAGTGGCCAGGAAAGGGGAAGAGAGAGAAAGAGAGAGAGACGAAGGAGCGCGTCCCGTCGTTGTGCGTCTTCTGTGGTGGCGCATTTAAATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCATAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTT