pLEXSY_I-ble2   EGE-241

Description:

nt 1-734:		5īpart of odc gene of Leishmania tarentolae
nt 740-759:		T7 promoter
nt 766-786:		TET operator
nt 788-1168:		5īnon-translated region of aprt gene of Leishmania tarentolae 
			with splice acceptor site for target gene
nt 1184-1252: 		LMSAP signal peptide of L. mexicana  secreted acid phosphatase
nt 1253-2260		stuffer fragment to be replaced by target gene
nt 2261-2278		Hexa-His stretch coding region
nt 2290-3628:		1.4k intergenic region from cam operon of Leishmania tarentolae 
			with polyA site for target gene and splice acceptor site for marker gene 
nt 3640-4014:		ble marker gene
nt 4055-4628:		5'UTR of dhfr-ts gene of Leishmania with polyA site for marker gene
nt 4757-5538:		3īpart of odc gene of Leishmania tarentolae
nt 5539-8398:		pUC18 derived with ori and bla marker gene @ nt 6325-7182.


AAATGAATACTTACAAGGTACTCCTGCACCCGGCACAAGCGTATGTAATTGCTAGCACGCCGGTGCCGACCCAACCTCCAACTCCCGTGCTCACGCGCGCACACACGCACGCACAAGCATCATCATCATCATCATCATGGTCATCCCTCCCTTATCTGGCGAAGGAGGCACTGTGACGCAAGGTTTTCTGTGTCTCTCCTACCCCTCTACTGATTCCTTCTTTCTCTCTTCCTCTTCTCGCACTGCACCCCCCTCCCCCCCCCCACACACACACACACTTGCTGTCATGGCTGTGCGCATTCGCTCACTCTTGTCGTGTCCCGTTTCCCTCATGGCGTCGTCGTCTTCCCCTGCGCAACCACCCACGCTGCACACGCACGCTCCACAGCGCGCTTCGCCTTCTTCGCTCTCTCCCTAGCCTCCTCTCTGTCACCCTCCACTGCCGCCGCGGTTGGCCGCTCTGCATCCTTTTCCCGCAGCCTTTCCTTTTTTTTTTCACTGTCAGTCTGGCTCATACCCGTGGAGCTGTGTCTCCCCGAGCTTCCCATACACCCACCCCCCACGACCTTTCTCAGCTTCCTTTCTCAGTCGCTTGAGCACGCAGTGCATTCACTTCTTCGTCCCTCAGTTCGCCTCGCTCTCGCTCTCTCTCGCGCGCGCTGCCGTACCCGCCCCTCCTCGTACTCCCCTCCTTCCTCATCCCCACCCCCCCCGTGTGCGCCGGCTCCTCTCTGAATTCTAATACGACTCACTATAGGGAGATCGtccctatcagtgatagaGATCCAGTCGCAGCCTGACCGCATCACACATCAAGgCGTTACAGCCTCTCCGTCTTTGCTGCACCCAAGtGTATTCGTGTGACCGCGTTGAGGGATCTAATGGATTCGGAACTTTGGTCGTTTGGTCTCTCATGCTGCCCAATCCCATGCGCTTTTCTCCGTGCCTCTCTGTCTCCCCTTCCGTTCTATTTGGACAACGATGTGTACTGTAATGcGTGCGCCATCTGACAACGAATAGATCAGCAgcATTCGCACACTTGCACATACCCAGTGAAGCTTTTGTGTCTGTCGTATTGACAACACCGACTGCAACAAGGTGTAGATAGAAGTTGGCCTTCTCGCTCGCTCGCACGCTCTTCACGCTCCTGCTTTCCTTGCTGTGCCTTgCCACCAgatctgCCATGGCcTCgAGGCTCGTCCGTGTGCTGGCcGCCGCCATGCTGGTTGCAGCGGCCGTGTCGGTCGACGCtGGCgCCtctctagacacaatatgtataggctaccatgcgaacaattcaaccgacactgttgacacagtgctcgagaagaatgtgacagtgacacactctgttaacctgctcgaagacagccacaacggaaaactatgtagattaaaaggaatagccccactacaattggggaaatgtaacatcgccggatggctcttgggaaacccagaatgcgacccactgcttccagtgagatcatggtActacattgtagaaacaccaaactctgagaatggaatatgttatccaggagatttcatcgactatgaggagctgagggagcaattgagctcagtgtcatcattcgaaagattcgaaatatttcccaaagaaagctcatggcccaaccacaacacaaAcaaaggagtaacggcagcatgctcccatgcggggGaaagcagtttttacagaaatttgctatggctgacggagaaggagggctcatacccaaagctgaaaaattcttatgtgaacaagaGagggaaagaagtccttgtactgtggggtattcatcacccgtctaacagtaaggaGcaacagaatatctatcagaatgaaaatgcttatgtctctgtagtgacttcaaattataacaggagatttaccccggaaatagcagaaagacccaaagtaagagatcaagctgggaggatgaactattactggaccttgctaaaacccggagacacaataatatttgaggcaaatggaaatctaatagcaccaaggtatgctttcgcactgagtagaggctttgggtccggcatcatcacctcaaacgcatcaatgcatgagtgtaacacgaagtgtcaaacacccctgggagctataaacagcagtctccctttccagaatatacacccagtcacaataggagagtgcccaaaatacgtcaggagtgccaaattgaggatggttacaggactaaggaacattccgtccattcaatccGCTAGCCTTAAGGGTACCCACCACCATCACCACCACTAGGCggccgcCCTCCTCCTCCTTTCTTGTTCCTTTCACGTCGCCTTCTCGGTTGTAGCTGGCAGACGACGAGTCTTACTTTTACGTGTACTTCTCTATAGATGATGTATGATCTCTCTGCATGCGTGTTCGTGCATGTGTCCGTGTGTTGTGTACGCGTGCGTCTCGCCTCAGCTCTCCGCGTGAAAGGGTTTGACTGCCCATGATGCGTGTGTATATCCACGCGCAGGCACGCACACACACACACAcaCACACACACACAggCACACACAGGCACACAAACGCATCTCAGGCCGAGCCGCATACGTCTCTCGCACGGTCTCGTTTATTTGATCATGTAGTTGAGTATTAAATTGGGAAGACAAAAACATAATAGCACGAAGAGTCGGGCACGAAAAGCCCGATCTCTCTCTCTCTCTCTCGTGCGCGCAGGGGCGTGTGGGTGCACGACGACGAGGACGGAGGGGGGAAGGGAGGCATAAACGgatcGATCCCCCATCGCACATGCGGGTACGAGCATTATCTGCTGTGTCTGGTCCTTTATCATATCGCTACCCGCCCGCCCCCCGCCCCCCTCCCCCCCCCCCCCCCGcGGcTcCTGTCACTGTCGTTCCTGCGACTCCCCACACACCCGCGCAACGCTCATGTCACGAAGAGAAAAGTAGAAGGCGTGGCGATGCGTTGCGCGGCCCCCTGTCCGCTGGCATGCAGACGTCGAGCCGTGGAACGGATGGTAGCGAGAGACAACGCGGCGATGCAGGAAAGGAGATTTACTGCAGGACGTCTACACACGCGCGCACACCACTGCGAATGGCACCGTGCTGAGGAAAATTGGGGGGGAGGGCGCACGGGGGCGGGGCAGGAAACGGTTGGATCAGCAGCACTCTCAACTTGTGTCCGTATTGCGAAGGAGGGAGAGGAGCCTGCCCGTATTATGGGCGCTGAAACGTCGGAACCACCTATGAATCCCACTTCTGCGTCGCGGAGGCGTACTGCTTCCGACCACCTCCGCAGCTCACGCGCGTCGAGCTCCCTCCCTTTCCCCTTCTTTCCGTTGTGTGTGTGTGTGTGTGTGTGTCTTGTCACGATGCATGGCCATCTCTCCTCCAACCCTCCACGCCTTCCTCTCCCCCGCCCATCAAACGCGCTACGGCCACAACCATTTCTTCAAGTATCACATCCACCACCACTCTTACCCACCTACCCTCTTCGACTCTGACACAGCCACTGACGCCCTCTCCGCCTCTCTCGCTCGCTGCACATTGACTCTCCACAGCCCCCCCTCCCTCGCACAGCCAGCCccacccacCCACCCACCCACCCACCGCTCTACACACCTCCACAGCAGCCgGATCCaccgCATGGCCAAGTTGACCAGTGCCGTTCCGGTGCTCACCGCGCGCGACGTCGCCGGAGCGGTCGAGTTCTGGACCGACCGGCTCGGGTTCTCCCGGGACTTCGTGGAGGACGACTTCGCCGGTGTGGTCCGGGACGACGTGACCCTGTTCATCAGCGCGGTCCAGGACCAGGTGGTGCCGGACAACACCCTGGCCTGGGTGTGGGTGCGCGGCCTGGACGAGCTGTACGCCGAGTGGTCGGAGGTCGTGTCCACGAACTTCCGGGACGCCTCCGGGCCGGCCATGACCGAGATCGGCGAGCAGCCGTGGGGGCGGGAGTTCGCCCTGCGCGACCCGGCCGGCAACTGCGTGCACTTCGTGGCCGAGGAGCAGGACTGACCGACTAGTTCTAGTTCTAGGGGcCGAATTAATTCAGATCCTCGTGTGAGCGTTCGCGGAATCGGTCGCTCGTGTTTATGCCCGTCTTGGTGTTGTGCTCGCAAGGCGGTGCAGCAGGATACCGTCGCCCTCCTCTCTCCTTGCTTCTCTGTTCTTCAATTCGCGATCTCACAGAGGCCGGCTGTGCACGCCCTTCCTCACCCTCCTTTTCCCACCTCTCGGCCACCGGTCGGCTCCGTTCCGTCTGCCGTCGAGAAGGGACGGGCATGTGCAGCTCCTCCCTTTCTCTCGCGCGCGCATCTTCTCTTGCTTGTGGCACTCACGCTCATGCGTCAAGGCGGCCCCACGcCGAGCCCCTGCGCTCCCTTCCCTCTTGCGCATCCGTAGCgcGgaTGccGTCGATGCGCAAGGCCGGCATGAAGGAGCGCGTGCCCTCAAGAggGCacACTATCATGCCCTACGTGGGCCACGCAGCGATGAGGCCGGCTTCgGCGGAGATGCGTCACGCACGTGCCAGATGATGCgcTACGCCTTCCTTGACTTGCGCCCCCCTCTCTTCCTCCGTCTCTCACTCTCTCTCTCTCACACACACACACACACACACACACACACAAAGCTCCGGTTCGTCCGGGCCCCTGCAGGCATGCCGACGTTCGGTGACCTGAACCACCTCGTCGCCGCTGTGATGTCTGGCGTGACCTGCTGCCTGCGCTTCCCTGGCCAGCTGAACTCTGACCTGCGCAAGCTTCTCCTGCTCAACGACGGCGACGGCGCTGACGAGGTGCAGAGTGGCACAGAAGCCGCAGCGGCGTACAGCGACGAGGAGGGCGGGAAGTCGCCGAGTGGTCCCCTGGCGAACGACTCCCTGTTCATGTCCGCGTGGGACCGCCGTCGCAACCTCACGAGCCGCCCGCTGCGCATCACAACCATATTCGGCCCCACCTGCGATTCGATGGACTGCATTCTGAAGAAGCAGCCCTTCCCGGAGATGAAGCTCGGCGACTGGCTTCTAGTGCCAGACATGGGCAGCTACACCACCGCTGCCGCGGGAGTCTTCAACGGTTTCGCGACACGCCGCCTTGAGTTTGTGAGCTCTGCTGACTTGTCTGCGAGGTCGAGGGCTGTGCATGCGCATGAGGGCAACACTTTTGAGTTTGTGAGCGAGTGAAAACGGGGCTGAGAGGATACAAGGTGCTGTAGGATGGGATCtTCCGCCCACCAGAGCATGGGCTATCTCTTGTGTCTGTTCTGAGCCTGCCTCTCTCTCTCTCTCTTGCCTCCCCCTCCCCCCTCCCCACACACACCCCACACGCACGCTCTGCGTCACCCTCTCCTCCCTCAGTAACGGGAGTAGACATGCAGAAGAAGTATACCACTAATACGTATGCGTTCCTGCAGGCATCCGAGATGGACACCAGAGCAAGCGCGGGTGTGCGCGAGTGGCCAGGAAAGGGGAAGAGAGAGAAAGAGAGAGAGACGAAGGAGCGCGTCCCGTCGTTGTGCGTCTTCTGTGGTGGCGCATTTAAATCCAATTCGCCCTATAGTGAGTCGTATTACGCGCGCTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTtAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGcGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGcCGCGCTTAATGCGCCGCTACAGGGCGCGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCGCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGATTT